细胞力动力学的定量、无标签映射

IF 13 2区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Small Pub Date : 2025-06-01 DOI:10.1002/smll.202501394

Xinyu Zhou, Ryan Porter, Xiaoyan Zhou, Jiapei Jiang, Brandyn Braswell, Zijian Wan, Pengfei Zhang, Shaopeng Wang

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引用次数: 0

摘要

无标签等离子体细胞力显微镜是发展揭示细胞施加的力在衍射有限的空间分辨率。通过等离子体散射成像实时量化细胞-基质相互作用动力学，可以准确地绘制细胞力的时空演变。为了证明该技术的能力，心肌细胞的力量演变和负荷率以毫秒分辨率测量。此外，对烟碱受体激活的细胞力反应进行了监测，并观察了细胞群中异质细胞力的变化，强调了这种无标记方法的多功能性和潜在影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Quantitative, Label-Free Mapping of Cell Force Dynamics

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Quantitative, Label-Free Mapping of Cell Force Dynamics

Label-free plasmonic cell force microscopy is developed to reveal cell exerted force at diffraction-limited spatial resolution. By quantifying cell-substrate interaction dynamics in real-time through plasmonic scattering imaging, the spatial and temporal evolutions of cellular forces are accurately mapped. To demonstrate the capability of the technology, cardiomyocyte force evolution and loading rates are measured with millisecond resolution. Furthermore, cell force responses to nicotinic receptor activation are monitored and observed heterogenic cell force changes among a population of cells, underscoring the versatility and potential impact of this label-free approach.

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来源期刊

Small 工程技术-材料科学：综合

CiteScore

17.70

自引率

3.80%

发文量

1830

审稿时长

2.1 months

期刊介绍： Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments. With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology. Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.