Wei Zhang, Yan Ge, Lihe Yao, Qingchun Yan, Jiuju Wei, Yanfei Yin, Bin Liu
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The meta-analysis was performed using the Stata MP16 software.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>A total of 64 studies were included, with a meta-analysis of the diversity index performed on a subset of seven studies. Microbial diversity of patients infected with HPV was observed to be significantly different from that of healthy controls (CHAO index: 95% CI 0.42, 5.03, <i>I</i><sup>2</sup> = 99.18%, <i>p</i> < 0.05). Subgroup analysis based on the sample collection region showed a significant difference between vaginal microbiota of the treatment group and control group, as measured by the Shannon index (95% CI 0.12, 0.97, <i>I</i><sup>2</sup> = 67.09%, <i>p</i> < 0.05). Further, subgroup analysis of samples sequenced with the primer pair for the V3–V4 region showed a statistically significant difference in alpha diversity (Shannon index: 95% CI 0.28, 0.72, <i>I</i><sup>2</sup> = 0.00%, <i>p</i> < 0.05) between treatment and control groups. The microbial diversity varied between patients with inferior cervical lesions (low-grade squamous intraepithelial lesion) and healthy controls (Shannon index: 95% CI 0.02, 0.58, <i>I</i><sup>2</sup> = 0.00%, <i>p</i> < 0.05). The bacterial marker genera differed at each cervical lesion stage. <i>Gardnerella</i> was prevalent during the HPV infection stage, but its proportion decreased after the occurrence of cervical lesions. In contrast, the proportions of <i>Prevotella</i>, <i>Porphyromonas</i>, and <i>Dialister</i> increased during the cervical cancer stages.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Patients with simple HPV infections frequently exhibit unstable microbial diversity and are influenced by various factors. The microbial environment continues to change after the occurrence of cervical lesions and is correlated with the severity of cervical lesions.</p>\n </section>\n </div>","PeriodicalId":9440,"journal":{"name":"Cancer reports","volume":"8 6","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cnr2.70246","citationCount":"0","resultStr":"{\"title\":\"Changes of Microbiome in Human Papillomavirus Infection and Cervical Cancer: A Systematic Review and Meta-Analysis\",\"authors\":\"Wei Zhang, Yan Ge, Lihe Yao, Qingchun Yan, Jiuju Wei, Yanfei Yin, Bin Liu\",\"doi\":\"10.1002/cnr2.70246\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background and Objectives</h3>\\n \\n <p>We aimed to conduct a systematic review and meta-analysis of high-throughput sequencing studies to assess changes in microbiome alpha, beta diversity, and composition differences in patients with human papillomavirus (HPV) infection and cervical cancer.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>The PubMed, Embase, Web of Science, and Cochrane Library databases were systematically searched to include original studies. 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引用次数: 0
摘要
背景和目的本研究旨在对高通量测序研究进行系统回顾和荟萃分析，以评估人乳头瘤病毒（HPV）感染和宫颈癌患者微生物组α、β多样性的变化和组成差异。方法系统检索PubMed、Embase、Web of Science和Cochrane Library数据库，纳入原始研究。95%置信区间的效应量估计值使用随机效应模型进行组合。meta分析采用Stata MP16软件进行。结果共纳入64项研究，并对7项研究的子集进行了多样性指数的荟萃分析。HPV感染患者的微生物多样性与健康对照组有显著差异（CHAO指数：95% CI 0.42, 5.03, I2 = 99.18%, p < 0.05）。基于样本采集区域的亚组分析显示，治疗组与对照组阴道微生物群的Shannon指数差异有统计学意义（95% CI 0.12, 0.97, I2 = 67.09%, p < 0.05）。此外，对V3-V4区引物对测序的样品进行亚组分析显示，处理组与对照组之间α多样性差异有统计学意义（Shannon指数：95% CI 0.28, 0.72, I2 = 0.00%, p < 0.05）。下宫颈病变（低级别鳞状上皮内病变）患者与健康对照组的微生物多样性存在差异（Shannon指数：95% CI 0.02, 0.58, I2 = 0.00%, p < 0.05）。宫颈病变各阶段细菌标记属不同。Gardnerella在HPV感染阶段流行，但在宫颈病变发生后比例下降。相比之下，普雷沃氏菌，卟啉单胞菌和Dialister的比例在宫颈癌阶段增加。结论单纯HPV感染患者微生物多样性不稳定，受多种因素影响。宫颈病变发生后，微生物环境持续变化，与宫颈病变的严重程度相关。
Changes of Microbiome in Human Papillomavirus Infection and Cervical Cancer: A Systematic Review and Meta-Analysis
Background and Objectives
We aimed to conduct a systematic review and meta-analysis of high-throughput sequencing studies to assess changes in microbiome alpha, beta diversity, and composition differences in patients with human papillomavirus (HPV) infection and cervical cancer.
Methods
The PubMed, Embase, Web of Science, and Cochrane Library databases were systematically searched to include original studies. The effect size estimates with a 95% confidence interval were combined using a random effects model. The meta-analysis was performed using the Stata MP16 software.
Results
A total of 64 studies were included, with a meta-analysis of the diversity index performed on a subset of seven studies. Microbial diversity of patients infected with HPV was observed to be significantly different from that of healthy controls (CHAO index: 95% CI 0.42, 5.03, I2 = 99.18%, p < 0.05). Subgroup analysis based on the sample collection region showed a significant difference between vaginal microbiota of the treatment group and control group, as measured by the Shannon index (95% CI 0.12, 0.97, I2 = 67.09%, p < 0.05). Further, subgroup analysis of samples sequenced with the primer pair for the V3–V4 region showed a statistically significant difference in alpha diversity (Shannon index: 95% CI 0.28, 0.72, I2 = 0.00%, p < 0.05) between treatment and control groups. The microbial diversity varied between patients with inferior cervical lesions (low-grade squamous intraepithelial lesion) and healthy controls (Shannon index: 95% CI 0.02, 0.58, I2 = 0.00%, p < 0.05). The bacterial marker genera differed at each cervical lesion stage. Gardnerella was prevalent during the HPV infection stage, but its proportion decreased after the occurrence of cervical lesions. In contrast, the proportions of Prevotella, Porphyromonas, and Dialister increased during the cervical cancer stages.
Conclusions
Patients with simple HPV infections frequently exhibit unstable microbial diversity and are influenced by various factors. The microbial environment continues to change after the occurrence of cervical lesions and is correlated with the severity of cervical lesions.
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