高致病性交替稻瘟病菌DT-DYLC对Chenopodium album L的诱变及转录组学分析

IF 2.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yuzhe Wang, Haiyang Cheng, Hongyu Cheng, Juan Li, Xin Wang, Haixia Zhu, Youhai Wei, Liang Cheng
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引用次数: 0

摘要

生物除草剂的发展往往受到除草活性低的限制。为了解决这一问题,我们采用紫外诱变和亚硝酸盐诱变联合的方法,增强了一种生物防治菌株alternnaria alternata DT-DYLC对Chenopodium album L.的毒力。突变株J在离叶试验中的致病性比野生型高2.4倍,在盆栽试验中的致病性比野生型高7.19%。感染期间的转录组分析显示有3060个差异表达基因(deg),其中257个与代谢过程有关。值得注意的是,87个共表达的上调基因在细胞壁降解酶(CWDEs)、真菌毒素合成和有毒化合物运输中富集,表明突变体j的毒力机制增强。该研究表明,经典诱变有效提高了真菌的致病性,而转录组学对毒力相关途径的深入了解为优化生物除草剂菌株和了解草霉的致病生物学提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mutagenesis and transcriptomic analysis of a highly pathogenic Alternaria alternata DT-DYLC against Chenopodium album L.

The development of bioherbicides is often limited by low herbicidal activity. To address this challenge, we enhanced the virulence of Alternaria alternata DT-DYLC, a biocontrol strain against Chenopodium album L., through combined UV and nitrite mutagenesis. Mutant strain J exhibited 2.4-fold higher pathogenicity in detached-leaf assays and 7.19% greater efficacy in pot trials compared to the wild-type strain. Transcriptome analysis during infection revealed 3060 differentially expressed genes, with 257 linked to metabolic processes. Notably, 87 co-expressed up-regulated genes were enriched in cell wall degradation enzymes, fungal toxin synthesis, and toxic compound transport, suggesting enhanced virulence mechanisms in mutant J. This study demonstrates that classical mutagenesis effectively improves fungal pathogenicity, while transcriptomic insights into virulence-related pathways provide a foundation for optimizing bioherbicide strains and understanding A. alternata's pathogenic biology.

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来源期刊
Letters in Applied Microbiology
Letters in Applied Microbiology 工程技术-生物工程与应用微生物
CiteScore
4.40
自引率
4.20%
发文量
225
审稿时长
3.3 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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