食管癌的肿瘤蛋白水解活性。

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-04-01 Epub Date: 2025-05-30 DOI:10.1007/s10517-025-06415-w

E I Goufman, N B Tikhonova, K B Gershkovich, A P Aleksankin, A A Stepanov, N V Nizyaeva, V V Bochkov, L M Mikhaleva, O V Kovaleva, I S Stilidi, N E Kushlinskii

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引用次数: 0

摘要

恶性肿瘤的生长伴随着纤溶酶活性的增加，纤溶酶是肿瘤发生的关键酶之一。IgG蛋白被纤溶酶水解后产生的片段具有暴露的c端赖氨酸残基，可以与纤溶酶重链结合。分离出高纯度的纤溶酶原（纤溶酶前体）和含有赖氨酸结合位点的重链。IgG片段的形成是由于它们在溶液中被纤溶蛋白水解的结果。采用固定化重链ELISA法，我们发现食管癌患者血清中IgG片段浓度高于健康供者。该方法灵敏度高（91%），特异度高（85%）。

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Tumor Proteolytic Activity in Esophageal Cancer.

The growth of malignant tumors is accompanied by an increase in plasmin activity, one of the key enzymes in oncogenesis. IgG proteolysis by plasmin yields fragments with exposed C-terminal lysine residues that can bind to plasmin heavy chain. Highly purified plasminogen (plasmin precursor) and heavy chains containing lysine-binding sites were isolated. The formation of IgG fragments as a result of their proteolysis by plasmin in solution was shown. Using ELISA with immobilized heavy chains, we found increased concentration of IgG fragments in the sera of patients with esophageal cancer in comparison with healthy donors. The test had high sensitivity (91%) and specificity (85%).

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.