一种生物电子免疫传感平台,包括夹在石墨烯片之间的金树突状3D结构,用于口腔癌检测。

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2025-06-16 Epub Date: 2025-05-30 DOI:10.1021/acsabm.5c00368
Buddhadev Purohit, Rohini Kumari, Ananya Srivastava, Pranjal Chandra
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引用次数: 0

摘要

流行病学研究表明,口腔癌是最致命的癌症之一,远处转移和最终死亡的风险很高,主要是由于诊断较晚。口腔癌的无创诊断可以简化样本采集,与单步检测相结合,可以促进和普及早期诊断,最终降低死亡率。鉴于此,我们设计了一种简单、无创的免疫传感器,用于在临床安全范围和病变范围内轻松、选择性地鉴定唾液样本中的口腔癌生物标志物细胞角蛋白19片段21-1 (cyfr -21-1)。在被用作口腔癌标志物之前,cyfr -21-1被用作非小细胞肺癌(NSCLC)和其他癌症的生物标志物。由于其唾液分泌旺盛,近几十年来已成为早期发现口腔癌的有力而可靠的标志物;然而,很少有关于它的研究。此外,文献报道唾液中cyfr -21-1标记物的敏感性和特异性高于血清样本。因此,在本研究中,利用夹在氧化石墨烯(GO)薄片之间的金纳米枝晶(AuNDs)纳米复合材料开发了传感表面,用于灵敏检测唾液样本中的cyfr -21-1。基于纳米杂化体系的金属枝晶具有惊人的导电和催化性能。第一层氧化石墨烯作为底物促进AuNDs的生长,第二层氧化石墨烯通过生物偶联反应与抗cyfr -21-1抗体共价结合。开发的生物电子免疫传感平台能够在临床安全范围内检测cyfr -21-1,并且具有低检测限(LOD)。它易于操作,可与便携式电化学分析仪集成,用于低资源环境下的即时口腔癌诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Bioelectronic Immunosensing Platform Comprising a Gold Dendritic 3D Architecture Sandwiched between Graphene Sheets for Oral Cancer Detection.

Epidemiological studies have shown that oral cancer is one of the most lethal forms of cancer, with a high risk of distant metastases and eventual death, largely due to late diagnosis. The noninvasive diagnosis of oral cancer can simplify sample collection, and when paired with single-step detection assays, it can facilitate and popularize early diagnosis, ultimately reducing the mortality rate. In light of this, we have designed a simple and noninvasive immunosensor for the facile and selective identification of the oral cancer biomarker cytokeratin 19 fragment-21-1 (CYFRA-21-1) in saliva samples, specifically in the clinically safe as well as diseased range. Prior to being utilized as a marker for oral carcinomas, CYFRA-21-1 was used as a biomarker for nonsmall cell lung cancer (NSCLC) as well as other cancers. Due to its high salivary secretion, it has become a powerful and reliable marker for the early detection of oral cancer in recent decades; however, there are very few studies pertaining to it. Moreover, the literature has reported the higher sensitivity and specificity of CYFRA-21-1 markers in saliva than in serum samples. Hence, in this study, the sensing surface was developed using a nanocomposite of gold nanodendrites (AuNDs) sandwiched between graphene oxide (GO) sheets for the sensitive detection of CYFRA-21-1 in saliva samples. Metallic dendrites based on nanohybrid systems possess astonishing conductive and catalytic properties. The first layer of GO is used as the substrate for enhanced growth of AuNDs, while the second layer of GO is utilized for covalent binding of the anti-CYFRA-21-1 antibody through bioconjugation reactions. The developed bioelectronic immunosensing platform is able to sense CYFRA-21-1 in a clinically safe as well as diseased range with a low limit of detection (LOD). It is easy to operate and can be integrated with a portable electrochemical analyzer for point-of-care (POC) oral cancer diagnosis in low-resource settings.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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