透明质酸包被脂质体增强经皮药物传递和乳腺癌球体穿透

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-26 DOI:10.1016/j.colsurfb.2025.114817

Muhammad Umer Rajput , Syed Wajahat Ali , Wajiha Kainat , Sahar Madani , Weiping Ding

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引用次数: 0

摘要

经皮给药系统提供了一种非侵入性的给药途径，通过皮肤提供了治疗药物的可控和持续释放。本研究合成了透明质酸修饰的装载紫杉醇的质体（HA-ES-PTX）和装载PTX纳米粒子的质体（ES-PTX），用于经皮肤治疗癌症。采用薄膜水合法制备了ES-PTX，并通过静电吸引将HA-ES-PTX包覆，使透明质酸（HA）吸附在阳离子质体表面。用透射电镜（TEM）、DLS （DLS）、纳米滴分光光度计（nanodrop分光光度计）对其进行了形态学研究。细胞摄取研究表明，透明质酸修饰的钙黄蛋白修饰体（HA-ES-Calcein）比未修饰的钙黄蛋白修饰体（ES-calcein）和游离钙黄蛋白更内化。对乳腺癌4T1和MDA-MB-231细胞株的MTT实验表明，HA-ES-PTX比ES-PTX和游离PTX具有更强的细胞毒性。体外大鼠皮肤Franz扩散池实验表明，与ES-PTX和氢乙醇PTX相比，HA-ES-PTX具有更高的渗透能力。最后，利用MCF-7和HFL1细胞系构建乳腺三维肿瘤球体。将ES-PTX和HA-ES-PTX引入椭球体，并在5天内观察其大小。结果表明，HA-ES-PTX提供了一种安全高效的经皮给药系统，对乳腺三维肿瘤球体具有明显的抗肿瘤作用。

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Enhanced transdermal drug delivery and breast cancer spheroid penetration using hyaluronic acid-coated ethosomes

Transdermal drug delivery systems offer a non-invasive route for administering medications, providing a controlled and sustained release of therapeutic agents through the skin. Here, the hyaluronic acid modified ethosomes loaded with paclitaxel (HA-ES-PTX) and ethosomes loaded with PTX (ES-PTX) nanoparticles were synthesized for treating cancer via skin. The ES-PTX were successfully prepared by the thin film hydration method, and then HA-ES-PTX were coated through electrostatic attraction, which caused the attraction of hyaluronic acid (HA) on the surface of cationic ethosomes. Morphological studies by TEM, particle size/zeta potential by DLS, and encapsulation efficiency by a nanodrop spectrophotometer were conducted. Cell uptake studies showed that hyaluronic acid-modified ethosomes loaded with calcein (HA-ES-Calcein) were more internalized than non-modified ethosomes (ES-calcein) and free calcein. MTT assay on breast cancer 4T1 and MDA-MB-231 cell lines revealed that HA-ES-PTX was more cytotoxic than ES-PTX and free PTX. In vitro, Franz diffusion cell experiments with rat skin indicated HA-ES-PTX had increased penetration compared to ES-PTX and hydroethanolic PTX. Finally, 3D breast tumor spheroids were constructed using MCF-7 and HFL1 cell lines. ES-PTX and HA-ES-PTX were introduced into the spheroids, and their sizes were visualized over five days. The results indicated that HA-ES-PTX offers a safe and efficient transdermal drug delivery system and provides significant anti-tumor effects on the 3D breast tumor spheroid.

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.