3D打印微流控设备集成免疫亲和萃取,固相萃取,早产生物标志物荧光标记。

IF 6.2
Precision Chemistry Pub Date : 2025-03-03 eCollection Date: 2025-05-26 DOI:10.1021/prechem.4c00092
James D Holladay, Zachary A Berkheimer, Michael K Haggard, Jacob B Nielsen, Gregory P Nordin, Adam T Woolley
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引用次数: 0

摘要

一个小型的,基于生物标志物的早产(PTB)风险诊断将需要多个样品制备步骤集成在一个平台上。为此,我们创建了一个3D打印的微流体装置,结合了免疫亲和萃取(IAE),固相萃取(SPE)和荧光标记。该设备使用抗体功能化的IAE单体来选择性地提取PTB生物标志物,使用甲基丙烯酸十二酯反相SPE单体来浓缩和促进PTB生物标志物的荧光标记,使用3D打印阀门来控制通过单体的流量。实现功能器件的有利迭代设计过程被记录下来。IAE/SPE装置可选择性地、可重复地从缓冲液和耗尽的母体血清中提取三种PTB生物标志物,证明其在单一生物标志物和多重提取方面的实用性。在串联提取和荧光标记后,生物标记物从SPE整体中浓缩洗脱,便于未来与下游分析技术(包括微芯片电泳)集成。该装置有效地结合并自动化了正交色谱提取方法,为构建完整的微流控PTB预测平台迈出了实质性的一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
3D Printed Microfluidic Devices for Integrated Immunoaffinity Extraction, Solid-Phase Extraction, and Fluorescent Labeling of Preterm Birth Biomarkers.

A miniaturized, biomarker-based diagnostic for preterm birth (PTB) risk will require multiple sample preparation steps to be integrated in a single platform. To this end, we created a 3D printed microfluidic device that combines immunoaffinity extraction (IAE), solid-phase extraction (SPE), and fluorescent labeling. This device uses an antibody-functionalized IAE monolith to selectively extract PTB biomarkers, a lauryl methacrylate reverse-phase SPE monolith to concentrate and facilitate fluorescent labeling of PTB biomarkers, and 3D printed valves to control flow through the monoliths. The advantageous iterative design process for arriving at a functional device is documented. The IAE/SPE device performed selective, reproducible extractions of three PTB biomarkers from buffer and depleted maternal blood serum, demonstrating its utility for single-biomarker and multiplexed extractions. After tandem extraction and fluorescent labeling, biomarkers eluted from the SPE monolith in a concentrated plug, facilitating future integration with downstream analysis techniques including microchip electrophoresis. This device effectively combines and automates orthogonal chromatographic extraction methods and constitutes a substantial step toward a complete microfluidic PTB prediction platform.

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来源期刊
Precision Chemistry
Precision Chemistry 精密化学技术-
CiteScore
0.80
自引率
0.00%
发文量
0
期刊介绍: Chemical research focused on precision enables more controllable predictable and accurate outcomes which in turn drive innovation in measurement science sustainable materials information materials personalized medicines energy environmental science and countless other fields requiring chemical insights.Precision Chemistry provides a unique and highly focused publishing venue for fundamental applied and interdisciplinary research aiming to achieve precision calculation design synthesis manipulation measurement and manufacturing. It is committed to bringing together researchers from across the chemical sciences and the related scientific areas to showcase original research and critical reviews of exceptional quality significance and interest to the broad chemistry and scientific community.
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