Prevalence of the Histological Characteristics in Various Teeth of Different Age-groups: An Observational In Vitro Study.

Aim: To assess the prevalence and distribution of enamel lamellae, enamel tufts, and incremental lines in different tooth types (incisors, canines, premolars, and molars) across various age-groups (≤20, 21-40, 41-60, and 60 and above) using histological analysis.

Materials and methods: A total of 200 extracted human permanent teeth from individuals were collected and categorized into four groups, based on age-group I (≤20 years), group II (21-40 years), group III (41-60 years) and group IV (>60 years) including various tooth types such as incisor, canine, premolar and molars, respectively. Teeth were manually sectioned longitudinally using a carborundum disk and examined under a light microscope (10× magnification) to assess enamel lamellae, enamel tufts, enamel spindles, interglobular dentin, the cementoenamel junction (CEJ), and incremental lines in cementum. Data were analyzed using the Chi-square (χ²) test to determine statistical significance.

Results: Molars were the most examined teeth (37.0%), followed by premolars (25.5%), canines (22.5%), and incisors (15.0%). Enamel lamellae were mostly grade II (43.3%) with no significant variation by age (p = 0.261) or tooth type (p = 0.653). Type A enamel lamellae (91.5%) did not differ significantly by tooth type (p = 0.237) or age (p = 0.871). The extent was primarily type E2 (72.0%) with no significant differences (p > 0.05). Enamel tufts were mostly type M1/3 (71.5%) with no age (p = 0.313) or tooth type association (p = 0.413). Enamel spindles (79%) increased with age (p = 0.011) and with tooth type (p = 0.029). Interglobular dentin (77.5%) increased significantly with age (p < 0.001) but not tooth type (p = 0.871). GAP type was the most common CEJ type (55.5%) with significant differences (p < 0.05).

Conclusion: The study concluded that enamel lamellae and tufts showed no significant variation with age or tooth type. Enamel spindles increased with age and tooth type, while interglobular dentin increased with age but not tooth type. The most common CEJ type was GAP, with significant differences observed.

Clinical significance: Understanding the histological characteristics of enamel lamellae, tufts, and incremental lines in different teeth and age-groups is crucial in endodontics, as these structural variations influence crack propagation, fracture resistance, and treatment outcomes. Age-related changes affect dentin permeability, irrigation efficiency, and obturation success. These advancements play a crucial role in improving restorative and endodontic approaches for enamel preservation and regeneration. How to cite this article: Gupta S, Bathla S, Astekar M. Prevalence of the Histological Characteristics in Various Teeth of Different Age-groups: An Observational In Vitro Study. J Contemp Dent Pract 2025;26(2):155-162.