缩放DNA工程。

IF 14.9 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Trends in biotechnology Pub Date : 2025-10-01 Epub Date: 2025-05-28 DOI:10.1016/j.tibtech.2025.05.002

Weiyi Li, Po-Hsiang Hung, Takeshi Matsui, Sasha F Levy, Gavin Sherlock

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引用次数: 0

摘要

DNA可以被改造来生产新的生物制剂、基因疗法和细胞疗法，并对生物体进行重新编程。拥有大规模设计DNA的能力可以加速这些应用的发展。现有技术擅长通过化学或酶的方法合成短的寡核苷酸（可达2000 bp）和中等大小的DNA组装（可达5-7 kb）。然而，由于技术和成本障碍，大规模合成经过序列验证的更长的DNA （bbb10 kb）和/或构建高度复杂的组合DNA文库仍然是一个重大挑战。受最近对体内DNA处理平台的研究启发，用于兆碱基长DNA组装和高通量序列验证，我们讨论了如何使用这些平台来实现大规模的DNA工程。

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Scaling DNA engineering.

DNA can be engineered to produce new biologics, gene therapies, and cellular therapies, and to reprogram organisms. Having the ability to engineer DNA at scale can accelerate the development of these applications. Existing technologies excel at short oligonucleotide synthesis by chemical or enzymatic methods (up to 2000 bp) and intermediate-size DNA assembly (up to 5-7 kb). Yet synthesizing sequence-validated longer DNA (>10 kb) and/or constructing highly complex combinatorial DNA libraries at scale remains a significant challenge, due largely to technical and cost barriers. Inspired by recent studies on an in vivo DNA processing platform for megabase-long DNA assembly and on high-throughput sequence verification, we discuss how these platforms may be used to achieve DNA engineering at scale.

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来源期刊

Trends in biotechnology 工程技术-生物工程与应用微生物

CiteScore

28.60

自引率

1.20%

发文量

198

审稿时长

1 months

期刊介绍： Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).