{"title":"黄芪通过调节大鼠FXR/CYP7A1、ERK/p38 MAPK和TLR4/MyD88/NF-κB信号通路减轻肝纤维化。","authors":"Chu-Ling Zhang, Hai-Yan Yang, Jia-Le Yi, Meng-Ke Song, Zhao-Da Yu, Jia-Yu Huang, Li-Tao Yi, Min Li, Ji-Xiao Zhu","doi":"10.1093/jpp/rgaf022","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Fragaria orientalis Losinsk is widely used in Tibetan medicine to remove and eliminate yellow water, a symptom of visceral abscesses including hepatitis, suggesting that Fragaria orientalis may exert potential therapeutic benefits for liver conditions. This study aimed to elucidate the hepatoprotective mechanisms of Fragaria orientalis extract against hepatic fibrosis in rats, focusing on the FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB signaling pathways.</p><p><strong>Methods: </strong>Chemical profiling was measured by UPLC-Q-TOF/MS, followed by HPLC fingerprint. Hepatic fibrosis was induced in rats treated with 40% CCl4. Subsequently, the anti-fibrotic effects of Fragaria orientalis involved in different signaling pathways were examined.</p><p><strong>Results: </strong>Administration of Fragaria orientalis extract reduced serum levels of ALT, AST, ALP, TNF-α, IL-1β, and IL-6, as well as hepatic MDA, while increasing GSH-Px and SOD in liver homogenates. UPLC analysis identified key constituents, including quercetin-3-O-glucuronide and ellagic acid, contributing to its hepatoprotective profile. The extract modulated the FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB pathways, supporting its role in attenuating liver fibrosis.</p><p><strong>Conclusion: </strong>In conclusion, these findings suggest that Fragaria orientalis possesses hepatoprotective effects against hepatic fibrosis, likely through modulating oxidative stress, inflammation, and bile acid regulation pathways.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fragaria orientalis attenuates hepatic fibrosis through modulation of FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB signaling in rats.\",\"authors\":\"Chu-Ling Zhang, Hai-Yan Yang, Jia-Le Yi, Meng-Ke Song, Zhao-Da Yu, Jia-Yu Huang, Li-Tao Yi, Min Li, Ji-Xiao Zhu\",\"doi\":\"10.1093/jpp/rgaf022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Fragaria orientalis Losinsk is widely used in Tibetan medicine to remove and eliminate yellow water, a symptom of visceral abscesses including hepatitis, suggesting that Fragaria orientalis may exert potential therapeutic benefits for liver conditions. This study aimed to elucidate the hepatoprotective mechanisms of Fragaria orientalis extract against hepatic fibrosis in rats, focusing on the FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB signaling pathways.</p><p><strong>Methods: </strong>Chemical profiling was measured by UPLC-Q-TOF/MS, followed by HPLC fingerprint. Hepatic fibrosis was induced in rats treated with 40% CCl4. Subsequently, the anti-fibrotic effects of Fragaria orientalis involved in different signaling pathways were examined.</p><p><strong>Results: </strong>Administration of Fragaria orientalis extract reduced serum levels of ALT, AST, ALP, TNF-α, IL-1β, and IL-6, as well as hepatic MDA, while increasing GSH-Px and SOD in liver homogenates. UPLC analysis identified key constituents, including quercetin-3-O-glucuronide and ellagic acid, contributing to its hepatoprotective profile. The extract modulated the FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB pathways, supporting its role in attenuating liver fibrosis.</p><p><strong>Conclusion: </strong>In conclusion, these findings suggest that Fragaria orientalis possesses hepatoprotective effects against hepatic fibrosis, likely through modulating oxidative stress, inflammation, and bile acid regulation pathways.</p>\",\"PeriodicalId\":16960,\"journal\":{\"name\":\"Journal of Pharmacy and Pharmacology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pharmacy and Pharmacology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1093/jpp/rgaf022\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmacy and Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/jpp/rgaf022","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
摘要
目的:藏药中广泛使用东花草去除黄水,黄水是内脏脓肿包括肝炎的症状,提示东花草可能对肝脏疾病有潜在的治疗作用。本研究旨在从FXR/CYP7A1、ERK/p38 MAPK、TLR4/MyD88/NF-κB信号通路的角度,探讨香菜提取物对大鼠肝纤维化的保护作用机制。方法:采用UPLC-Q-TOF/MS进行化学谱分析,HPLC指纹图谱分析。40% CCl4诱导大鼠肝纤维化。随后,我们研究了不同信号通路中侧枝草的抗纤维化作用。结果:藿香提取物降低大鼠血清ALT、AST、ALP、TNF-α、IL-1β、IL-6及肝脏MDA水平,升高肝脏匀浆组织中GSH-Px、SOD水平。UPLC分析确定了关键成分,包括槲皮素-3- o -葡萄糖醛酸和鞣花酸,有助于其保护肝脏。该提取物可调节FXR/CYP7A1、ERK/p38 MAPK和TLR4/MyD88/NF-κB通路,支持其减轻肝纤维化的作用。结论:综上所述,这些研究结果表明,银花可能通过调节氧化应激、炎症和胆汁酸调节途径,具有抗肝纤维化的肝保护作用。
Fragaria orientalis attenuates hepatic fibrosis through modulation of FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB signaling in rats.
Objectives: Fragaria orientalis Losinsk is widely used in Tibetan medicine to remove and eliminate yellow water, a symptom of visceral abscesses including hepatitis, suggesting that Fragaria orientalis may exert potential therapeutic benefits for liver conditions. This study aimed to elucidate the hepatoprotective mechanisms of Fragaria orientalis extract against hepatic fibrosis in rats, focusing on the FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB signaling pathways.
Methods: Chemical profiling was measured by UPLC-Q-TOF/MS, followed by HPLC fingerprint. Hepatic fibrosis was induced in rats treated with 40% CCl4. Subsequently, the anti-fibrotic effects of Fragaria orientalis involved in different signaling pathways were examined.
Results: Administration of Fragaria orientalis extract reduced serum levels of ALT, AST, ALP, TNF-α, IL-1β, and IL-6, as well as hepatic MDA, while increasing GSH-Px and SOD in liver homogenates. UPLC analysis identified key constituents, including quercetin-3-O-glucuronide and ellagic acid, contributing to its hepatoprotective profile. The extract modulated the FXR/CYP7A1, ERK/p38 MAPK, and TLR4/MyD88/NF-κB pathways, supporting its role in attenuating liver fibrosis.
Conclusion: In conclusion, these findings suggest that Fragaria orientalis possesses hepatoprotective effects against hepatic fibrosis, likely through modulating oxidative stress, inflammation, and bile acid regulation pathways.
期刊介绍:
JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.