x射线能量对人间充质干细胞γ - h2ax和53BP1病灶定量产量的影响

IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
M A Ignatov, A K Chigasova, A A Osipov, Yu A Fedotov, N Yu Vorobyeva, A N Osipov
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引用次数: 0

摘要

我们比较了在50,100和200kvp的峰值阳极电压下产生的x射线对培养的人间充质干细胞中参与修复关键辐射诱导的DNA损伤,双链断裂(γH2AX和53BP1)的蛋白的焦点(局部动态微团)数量的影响。根据实验得到的γ - h2ax和53BP1病灶数量变化的剂量曲线计算相对生物效应(RBE),结果表明,相对于200 kVp x射线标准辐射为1,100 kVp辐射的RBE为~1.15,50 kVp辐射的RBE为~1.50。所得结果表明,为了评估低能x射线的实际RBE,有必要进行深入的基础研究,并随后对权重因子值进行修正。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of X-Ray Energy on the Quantitative Yield of γH2AX and 53BP1 Foci in Human Mesenchymal Stem Cells.

We compared the influence of X-rays generated at the peak anode voltages of 50, 100, and 200 kVp on the quantitative yield of foci (localized dynamic microclusters) of proteins involved in repair critical radiation-induced DNA damage, double-strand breaks (γH2AX and 53BP1), in cultured human mesenchymal stem stromal cells. Calculations of the relative biological effectiveness (RBE) based on the experimentally obtained dose curves of changes in the number of γH2AX and 53BP1 foci indicate that relative to the standard 200 kVp X-ray radiation taken as 1, the RBE of 100 kVp radiation is ~1.15, and that of 50 kVp is ~1.50. The obtained results indicate the necessity for thorough fundamental studies for evaluation of the real RBE of low-energy X-rays with subsequent correction of the weighting factor values.

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来源期刊
Bulletin of Experimental Biology and Medicine
Bulletin of Experimental Biology and Medicine 医学-医学:研究与实验
CiteScore
1.50
自引率
14.30%
发文量
265
审稿时长
2 months
期刊介绍: Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.
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