根分泌物中的氨基酸诱导芽孢杆菌孢子萌发,增强根定植,促进植物生长

IF 5.7 2区 生物学
Lili Tao, Xinli Sun, Pascale B. Beauregard, Taimeng Tan, Yuling Zhang, Jiyu Xie, Guidong Huang, Nan Zhang, Youzhi Miao, Qirong Shen, Zhihui Xu, Ruifu Zhang
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引用次数: 0

摘要

芽孢杆菌是一种促进植物生长的根瘤菌,已经作为生物肥料被商业化;它们是理想的,因为这些物种形成的孢子可以稳定地储存很长时间。然而,为了使这些孢子充分发挥其有益的作用,它们必须发芽。根际中具体的发芽信号,特别是来自植物根分泌物的信号,在很大程度上仍然是未知的。研究了黄瓜不同生长状态下velezensis芽孢杆菌SQR9和枯草芽孢杆菌NCIB 3610孢子的萌发信号。我们鉴定了相应的发芽受体,并在芽孢杆菌种之间进行了生化比较。较大的植株更容易刺激孢子萌发。鉴定出l -异亮氨酸、l -鸟氨酸、l -缬氨酸、l -丝氨酸和β-丙氨酸5种氨基酸作为孢子萌发信号。这些氨基酸与细菌孢子混合施用可显著提高白僵菌SQR9的促黄瓜性能。两种芽孢杆菌对这些氨基酸的萌发受体均为GerA。枯草芽孢杆菌和白僵芽孢杆菌孢子萌发效率的差异可能与GerA配体识别位点的差异有关。枯草芽孢杆菌NCIB 3610的GerA在白僵菌SQR9中的表达提高了后者的孢子发芽率。我们的研究强调了氨基酸在调节芽孢杆菌孢子萌发和随后的植物根定植中的关键作用,强调了它们增强芽孢杆菌生物肥料功效的潜力。萌发受体的工程化是提高生物肥料菌株孢子萌发效率的一种很有前途的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amino Acids From Root Exudates Induce Bacillus Spore Germination to Enhance Root Colonisation and Plant Growth Promotion

Strains of Bacillus species, plant growth-promoting rhizobacteria, have been commercialised as biofertilisers; they are ideal for this because these species form spores that can be stored stably for a long time. However, for these spores to exert their full beneficial effects, they must germinate. The specific germination signals in the rhizosphere, particularly those from plant root exudates, remain largely unknown. Here, we investigated the germination signals from different growth states of cucumber (Cucumis sativus) for spores of Bacillus velezensis SQR9 and Bacillus subtilis NCIB 3610. We identified the corresponding germination receptors and compared them biochemically between the Bacillus species. Larger plants better stimulated spore germination. Five amino acids—L-isoleucine, L-ornithine, L-valine, L-serine and β-alanine were—identified as spore germination signals. Combined application of a mixture of these amino acids with bacterial spores markedly enhanced the cucumber growth-promoting properties of B. velezensis SQR9. The germination receptor for these amino acids was GerA in both Bacillus species. Differences in spore germination efficiency between B. subtilis and B. velezensis may be attributable to variations in the GerA ligand-recognition sites. Expression of GerA from B. subtilis NCIB 3610 in B. velezensis SQR9 enhanced the spore germination rate of the latter. Our study highlights the pivotal role of amino acids in regulating spore germination of Bacillus and subsequent plant root colonisation, emphasising their potential to enhance the efficacy of Bacillus-based biofertilisers. Engineering of germination receptors is a promising approach to enhance the spore germination efficiency of biofertiliser strains.

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来源期刊
Microbial Biotechnology
Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
11.20
自引率
3.50%
发文量
162
审稿时长
1 months
期刊介绍: Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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