跨种PCR和实时PCR法鉴定犬科种和亚种

IF 1.8 3区生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal genetics Pub Date : 2025-05-31 DOI:10.1111/age.70019

Aliaksandra E. Hrebianchuk, Nastassia S. Parfionava, Iosif S. Tsybovsky

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引用次数: 0

摘要

基于跨种扩增过程中微卫星位点的表现特征，提出了犬科4种物种——赤狐（Vulpes Vulpes）、北极狐（Vulpes lagopus）、貉（Nyctereutes procyonoides）和灰狼（Canis lupus lupus）和家犬（Canis lupus familiaris）的物种鉴定方法。此外，我们提出了一种基于淀粉酶基因拷贝数变化的狼犬、灰狼和家犬亚种的DNA鉴定方法。这项对生活在白俄罗斯的犬科动物的全面研究，使得在对个体进行遗传鉴定之前，能够可靠地根据物种和亚种区分法医样本。

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Forensic identification of species and subspecies of Canidae family by cross-species PCR and real-time PCR

We propose an approach to species identification of four species of the Canidae family, the red fox (Vulpes vulpes), the arctic fox (Vulpes lagopus), the raccoon dog (Nyctereutes procyonoides), and the wolf including the gray wolf (Canis lupus lupus) and the domestic dog (Canis lupus familiaris), based on characteristics of manifestation of microsatellite loci during cross-species amplification. Additionally, we present an approach to DNA identification of the subspecies of Canis lupus, the gray wolf and the domestic dog, based on variations in the copy number of the amylase gene. This comprehensive study of canids living in Belarus makes it possible to reliably differentiate forensic samples by species and subspecies before genetic identification of individuals.

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来源期刊

Animal genetics 生物-奶制品与动物科学

CiteScore

4.60

自引率

4.20%

发文量

115

审稿时长

5 months

期刊介绍： Animal Genetics reports frontline research on immunogenetics, molecular genetics and functional genomics of economically important and domesticated animals. Publications include the study of variability at gene and protein levels, mapping of genes, traits and QTLs, associations between genes and traits, genetic diversity, and characterization of gene or protein expression and control related to phenotypic or genetic variation. The journal publishes full-length articles, short communications and brief notes, as well as commissioned and submitted mini-reviews on issues of interest to Animal Genetics readers.