基于WGCNA和机器算法预测生姜靶向PRMT1/BTG2轴抑制胃癌的分子机制

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-05-24 DOI:10.1016/j.phymed.2025.156892

Guoqing Chen , Boyun Gou , Yuhua Du , Ziying Zhou , Yuting Bai , Yi Yang , Yi Nan , Ling Yuan

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引用次数: 0

摘要

目的通过网络药理学、WGCNA和机器算法筛选生姜抗胃癌的生物标志物。寻找构成信号轴的上游转录因子和下游信号蛋白，预测生姜抗胃癌可能的作用机制。方法通过公开数据库筛选生姜的有效成分和靶点。采用疾病数据库、GEO数据库和WGCNA对GC基因进行筛选。取这四个基因的交集来获得潜在的核心基因。采用机器算法筛选核心基因。对核心基因进行临床相关性分析、基因突变关系分析、表观遗传调控分析、免疫浸润分析和分子对接验证。通过数据库查找其上游转录因子和下游信号蛋白。结果通过数据库和WGCNA分析，共获得35个交叉基因。结合机器算法和PPI筛选核心基因PRMT1。通过数据库和分子对接鉴定PRMT1的上游转录因子为EGR1，下游转录蛋白为BTG2。结论通过网络药理学、WGCNA和机器算法，我们发现PRMT1可以作为生姜抗GC的生物标志物。我们推测生姜可能通过PRMT1/BTG2发挥抗肿瘤作用，为生姜抗胃癌的药理机制提供了新的思路。

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Predicting the molecular mechanism of ginger targeting PRMT1/BTG2 axis to inhibit gastric cancer based on WGCNA and machine algorithms

Objective

The aim of this study was to screen the biomarkers of ginger against gastric cancer (GC) by network pharmacology, WGCNA and machine algorithms. To find the upstream transcription factors and downstream signaling proteins constituting the signaling axis, so as to predict the possible mechanism of action of ginger against GC.

Methods

Ginger was screened for active ingredients and targets through public databases. GC genes were screened using disease database, GEO database and WGCNA. The intersection of the four was taken to obtain the potential core genes. Machine algorithms was used to screen the core genes. Clinical relevance analysis, gene mutation relationship, epigenetic regulation analysis, immune infiltration analysis and molecular docking validation were performed on the core genes. Find its upstream transcription factors and downstream signaling proteins through database.

Results

35 intersecting genes were obtained by databases and WGCNA analysis. Machine algorithms and PPI were combined to finally screen the core gene PRMT1. The upstream transcription factor of PRMT1 was identified as EGR1 and the downstream protein as BTG2 by database and molecular docking.

Conclusion

In this study, we found that PRMT1 could be used as a biomarker for ginger against GC using network pharmacology, WGCNA and machine algorithms. We hypothesized that ginger may exert antitumor effects through PRMT1/BTG2, providing new insights into the pharmacological mechanism of ginger against GC.

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.