亲水相互作用液相色谱-质谱联用分析甲基化季铵化合物。

IF 6.6 2区化学 Q1 SPECTROSCOPY

Mass Spectrometry Reviews Pub Date : 2025-05-29 DOI:10.1002/mas.21942

Iman Zarei, Ambrin Farizah Babu, Ville M Koistinen, Marjo Tuomainen, Anna Kårlund, Retu Haikonen, Marko Lehtonen, Kati Hanhineva

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引用次数: 0

摘要

液相色谱-质谱（LC-MS）是一种检测和定量生物基质中甲基化季铵化合物（mQACs）的强大技术，如酰基肉碱和甲基化氨基酸衍生（甜菜碱化）化合物。由于其高极性和永久电荷，mQACs提出了分析挑战，特别是在实现高效色谱保留和分辨率方面。本文主要研究了亲水相互作用液相色谱-质谱联用技术（HILIC-MS）在生物样品中对这类化合物的分析。我们强调了分析中的实际考虑因素，包括它们的MS/MS碎片模式和正电喷雾模式(ESI)+的鉴定，以支持研究人员在靶向或非靶向代谢组学研究中使用mQACs。

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Analysis of Methylated Quaternary Ammonium Compounds Using Hydrophilic Interaction Liquid Chromatography Combined With Mass Spectrometry.

Liquid chromatography-mass spectrometry (LC-MS) is a powerful technique for the detection and quantification of methylated quaternary ammonium compounds (mQACs), such as acylcarnitines and methylated amino-acid-derived (betainized) compounds, in biological matrices. Due to their high polarity and permanent charge, mQACs present analytical challenges, particularly in achieving efficient chromatographic retention and resolution. Here, we focus on the application of hydrophilic interaction liquid chromatography combined with mass spectrometric (HILIC-MS), for the analysis of these compound classes in biological samples. We highlight practical considerations in their analysis, including their MS/MS fragmentation patterns and identification in positive electrospray mode (ESI)⁺, to support researchers working with mQACs in targeted or untargeted metabolomics studies.

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来源期刊

Mass Spectrometry Reviews 物理-光谱学

CiteScore

16.30

自引率

3.00%

发文量

期刊介绍： The aim of the journal Mass Spectrometry Reviews is to publish well-written reviews in selected topics in the various sub-fields of mass spectrometry as a means to summarize the research that has been performed in that area, to focus attention of other researchers, to critically review the published material, and to stimulate further research in that area. The scope of the published reviews include, but are not limited to topics, such as theoretical treatments, instrumental design, ionization methods, analyzers, detectors, application to the qualitative and quantitative analysis of various compounds or elements, basic ion chemistry and structure studies, ion energetic studies, and studies on biomolecules, polymers, etc.