Investigation of the Effects of Solvents and Temperature on the Aggregation, Binding, and Phase Partitioning Nature of Triton X-100 with Promazine Hydrochloride

Herein, the aggregation, binding, and phase partitioning nature of triton X-100 (TX-100) with promazine hydrochloride (PMZ) have been examined applying spectroscopic and cloud point measurement methods. The PMZ mediated aggregation of TX-100 was found to be disfavored with the elevation of temperature. The binding constant (K_b) of the complexation of TX-100 with PMZ was evaluated applying Benesi–Hildebrand (BH) plot, where the K_b values showed the dependency on solvent composition and experimental temperature. Cloud point (CP) of TX-100 + PMZ mixture was noticed to be reliant on both concentration and solvent variation. The micellization and complexation of the TX-100 + PMZ system in all the investigated solvents were obtained as spontaneous with negative free energy changes $$ values, whereas clouding phenomenon was nonspontaneous ($$). For the TX-100 + PMZ system, the enthalpy changes ($$ and entropy changes $$ values indicated the presence of hydrophobic interaction, dipole–dipole forces and π–π interactions. The compensation parameters (intrinsic enthalpy gain ($$) and compensation temperature (T_c)) were also calculated and explained logically.