果胶和芽孢杆菌在罗虎饲料中联合施用：对罗虎生长、饲料利用、免疫、血液生化特性和病原体抗性的影响

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-05-30 DOI:10.1016/j.aqrep.2025.102885

Tanaya Sukul, Koushik Ghosh

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引用次数: 0

摘要

益生菌、益生元和合成菌已引起人们的关注，以提高鱼类的生长、免疫和抗病能力。本研究评估了果胶（1 %）和萨氏芽孢杆菌或解淀粉芽孢杆菌（7 Log CFU g−1饲料）对罗虎、罗氏Labeo rohita鱼种的单独或组合效果。对照组饲喂不含果胶和杆菌的基础饲粮(C)。在基础饲粮中添加果胶（E1）、萨伯氏芽孢杆菌（E2）、解淀粉芽孢杆菌（E3）、萨伯氏芽孢杆菌+ 果胶（E4）和解淀粉芽孢杆菌+ 果胶（E5），分3次饲喂试验鱼（6.4 ± 0.2 g），饲喂90 d。总体而言，结果表明，与对照组和仅使用益生菌/益生元饲料组相比，联合使用红曲霉和果胶（E4）显著提高了增重（%）和饲料效率（p <； 0.05）。饲粮中添加合成酶可提高消化酶（蛋白酶、脂肪酶）和糖酵解酶（葡萄糖激酶、己糖激酶）的活性。此外，与对照组相比，合成饲料组（E4和E5）在免疫参数（血清溶菌酶、抗蛋白酶、吞噬能力、呼吸爆发、过氧化物酶和补体活性）、红细胞、血红蛋白含量和血清蛋白方面表现出显著改善（p <； 0.05）。饲喂90 d后，将致病性sobriia气单胞菌腹腔注射到鱼体内，记录应激信息和免疫参数。结果表明，果胶与B. safensis或B.解淀粉酵母菌联合应用可提高侵染鱼的先天免疫和生存能力。此外，增加的超氧化物歧化酶和谷胱甘肽过氧化物酶活性可能表明鱼对氧化应激的恢复能力。饲粮中含有红曲霉和果胶的鱼具有最高的攻毒后存活率，这表明该特定组合可作为功能性饲料添加剂用于改善罗氏乳杆菌的生长、免疫和病原体抗性。

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Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance

Probiotics, prebiotics, and synbiotics have drawn attention to enhance growth, immunity, and disease resistance in fish. This study evaluated the single or combinatorial effects of pectin (1 %) and Bacillus safensis or B. amyloliquefaciens (7 Log CFU g⁻¹ feed) on rohu, Labeo rohita fingerlings. The basal diet without pectin or bacilli was the control (C). The basal diet fortified with pectin (E1), B. safensis (E2), B. amyloliquefaciens (E3), B. safensis + pectin (E4), and B. amyloliquefaciens + pectin (E5) were fed to the experimental fish (6.4 ± 0.2 g) for 90 days in triplicate. Overall, the results demonstrated significant (p < 0.05) improvement in weight gain (%) and feed efficiency with the combined application of B. safensis and pectin (E4) compared to the control and only probiotic/prebiotic fed groups. Improved activities of digestive (protease, lipase) and glycolytic enzymes (glucokinase, hexokinase) were noticed with dietary synbiotics administration. Further, synbiotics-fed groups (E4 and E5) demonstrated significant (p < 0.05) improvements in immune parameters (serum lysozyme, antiprotease, phagocytosis, respiratory burst, peroxidase, and complement activity), erythrocytes, haemoglobin content and serum protein compared to the control group. Following 90 days of feeding, pathogenic Aeromonas sobria was intra-peritoneally injected into the fish, and information on stress along with immune parameters was recorded. Results depicted that the application of pectin together with B. safensis or B. amyloliquefaciens improved the innate immunity and survivability of the challenged fish. Further, increased superoxide dismutase and glutathione peroxidase activities might indicate the fish's resilience to oxidative stress. Fish fed a diet containing B. safensis and pectin had the highest post-challenge survival rate, suggesting prospective application of this specific combination as functional feed additive to improve growth, immunity, and pathogen resistance in L. rohita.

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.