中国羊驼艾美耳球虫属（艾美耳球虫科）首次报道及三种羊驼艾美耳球虫的系统发育特征

IF 1.5 4区医学 Q3 PARASITOLOGY

Parasitology International Pub Date : 2025-05-28 DOI:10.1016/j.parint.2025.103097

Kaihui Zhang , Yixuan Niu , Jiashu Lang , Huijie Liu , Ziyang Qin , Yifan Zhang , Longxian Zhang

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引用次数: 0

摘要

为了解河南省郑州动物园家养羊驼艾美耳球虫的流行情况、分子特征及系统发育关系，采用毛囊糖浮法对25份新鲜粪便标本进行球虫检测。球虫总流行率为44.0%(11/25)，根据形态特征鉴定为羊艾美球虫、跛艾美球虫和普诺艾美球虫。采用单卵囊选择技术和多位点巢式PCR扩增技术，对艾美耳球虫的18S rRNA和COI位点序列进行分子生物学鉴定、同源性比较和系统发育分析。3种羊驼艾美耳球虫在18S rRNA和COI基因位点的序列同源性分别为98.8 ~ 99.4%和94.6 ~ 97.7%。在18S rRNA和COI位点上，3种艾美耳球虫与山羊和绵羊艾美耳球虫具有较高的同源性。据我们所知，本文首次报道了来自羊驼的3种艾美耳球虫的分子鉴定和进化分析。本研究也为羊驼艾美耳球虫提供了更为准确的鉴定方法。

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First report of Eimeria spp. (Eimeriidae) in alpaca (Lama glama) in China and phylogenetic characterization of three alpacan Eimeria species

To investigate the prevalence, molecular characteristics and phylogenetic relationship of Eimeria spp. domestic alpaca at the Zhengzhou Zoo, Henan Province, China, sheather's sugar floatation method was conducted to detect coccidia in 25 fresh fecal samples. The overall prevalence of coccidia was 44.0 % (11/25), Eimeria alpacae Guerrero (1967), Eimeria lamae Guerrero (1967), and Eimeria punoensis Guerrero (1967) were identified by their morphological characteristics. The 18S rRNA and COI loci sequences were sequenced for molecular biological identification, homology comparison, and phylogenetic analysis of Eimeria spp. by single oocyst selection technology and multi-locus nested PCR amplification. The sequence homology among the three Eimeria spp. from alpaca at the 18S rRNA and COI gene loci were 98.8–99.4 % and 94.6–97.7 %, respectively. At the 18S rRNA and COI loci, the three Eimeria spp. exhibited high identity with Eimeria spp. from goats and sheep. To our knowledge, this is the first report on the molecular identification and evolutionary analysis of three Eimeria spp. from alpaca. This study also provides a more accurate identification method for Eimeria spp. from alpaca.

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来源期刊

Parasitology International 医学-寄生虫学

CiteScore

4.00

自引率

10.50%

发文量

140

审稿时长

61 days

期刊介绍： Parasitology International provides a medium for rapid, carefully reviewed publications in the field of human and animal parasitology. Original papers, rapid communications, and original case reports from all geographical areas and covering all parasitological disciplines, including structure, immunology, cell biology, biochemistry, molecular biology, and systematics, may be submitted. Reviews on recent developments are invited regularly, but suggestions in this respect are welcome. Letters to the Editor commenting on any aspect of the Journal are also welcome.