采用微流控MEA技术对亚眶环境下三维神经元网络进行电生理研究。

IF 4.4 1区 物理与天体物理 Q1 MULTIDISCIPLINARY SCIENCES
Andie E Padilla, Gobinath C, Candice Hovell, Jeremy Mares, Veerle Reumers, Twyman Clements, Jason Rextroat, Paul Gamble, Ben Lumpp, Binata Joddar
{"title":"采用微流控MEA技术对亚眶环境下三维神经元网络进行电生理研究。","authors":"Andie E Padilla, Gobinath C, Candice Hovell, Jeremy Mares, Veerle Reumers, Twyman Clements, Jason Rextroat, Paul Gamble, Ben Lumpp, Binata Joddar","doi":"10.1038/s41526-025-00476-x","DOIUrl":null,"url":null,"abstract":"<p><p>Studying neuronal cells in space reveals how microgravity affects brain function, gene expression, and cellular processes. This study details the preparation and validation of a 3D neuronal electrophysiology (EPHYS) sensing microfluidic biodevice used during a suborbital space flight. Initially, the device's function was tested with rat hippocampal neurons using EPHYS data collected via a microelectrode array (MEA). This system was later applied to human glutamatergic (Glu) neurons for eight days preceding a suborbital flight. A live-dead assay confirmed cell viability, and the system was integrated into a CubeLab to maintain a controlled environment. Two biological samples were flown, along with two control samples, to validate the EPHYS system. Results showed that human Glu-neurons exposed to microgravity exhibited altered expression of vesicular glutamate transporters (VGLUTs) while maintaining neuronal differentiation markers. The findings contribute to understanding neurological disorders, neuro-inflammation, and cognitive impacts of space travel, with broader applications for brain health research on Earth.</p>","PeriodicalId":54263,"journal":{"name":"npj Microgravity","volume":"11 1","pages":"20"},"PeriodicalIF":4.4000,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117052/pdf/","citationCount":"0","resultStr":"{\"title\":\"Adoption of microfluidic MEA technology for electrophysiology of 3D neuronal networks exposed to suborbital conditions.\",\"authors\":\"Andie E Padilla, Gobinath C, Candice Hovell, Jeremy Mares, Veerle Reumers, Twyman Clements, Jason Rextroat, Paul Gamble, Ben Lumpp, Binata Joddar\",\"doi\":\"10.1038/s41526-025-00476-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Studying neuronal cells in space reveals how microgravity affects brain function, gene expression, and cellular processes. This study details the preparation and validation of a 3D neuronal electrophysiology (EPHYS) sensing microfluidic biodevice used during a suborbital space flight. Initially, the device's function was tested with rat hippocampal neurons using EPHYS data collected via a microelectrode array (MEA). This system was later applied to human glutamatergic (Glu) neurons for eight days preceding a suborbital flight. A live-dead assay confirmed cell viability, and the system was integrated into a CubeLab to maintain a controlled environment. Two biological samples were flown, along with two control samples, to validate the EPHYS system. Results showed that human Glu-neurons exposed to microgravity exhibited altered expression of vesicular glutamate transporters (VGLUTs) while maintaining neuronal differentiation markers. The findings contribute to understanding neurological disorders, neuro-inflammation, and cognitive impacts of space travel, with broader applications for brain health research on Earth.</p>\",\"PeriodicalId\":54263,\"journal\":{\"name\":\"npj Microgravity\",\"volume\":\"11 1\",\"pages\":\"20\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117052/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"npj Microgravity\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1038/s41526-025-00476-x\",\"RegionNum\":1,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"npj Microgravity","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/s41526-025-00476-x","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

在太空中研究神经元细胞揭示了微重力如何影响大脑功能、基因表达和细胞过程。本研究详细介绍了用于亚轨道太空飞行的3D神经元电生理(EPHYS)传感微流体生物装置的制备和验证。首先,通过微电极阵列(MEA)收集EPHYS数据,用大鼠海马神经元测试该装置的功能。该系统随后应用于人类谷氨酸(Glu)神经元,在亚轨道飞行前8天。活体实验证实了细胞活力,并将该系统集成到CubeLab中以保持受控环境。飞行了两个生物样本和两个对照样本,以验证EPHYS系统。结果显示,暴露于微重力环境下的人谷氨酸神经元在维持神经元分化标志物的同时,其囊泡谷氨酸转运体(VGLUTs)的表达发生改变。这些发现有助于理解神经系统疾病、神经炎症和太空旅行对认知的影响,并在地球上的大脑健康研究中有更广泛的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adoption of microfluidic MEA technology for electrophysiology of 3D neuronal networks exposed to suborbital conditions.

Studying neuronal cells in space reveals how microgravity affects brain function, gene expression, and cellular processes. This study details the preparation and validation of a 3D neuronal electrophysiology (EPHYS) sensing microfluidic biodevice used during a suborbital space flight. Initially, the device's function was tested with rat hippocampal neurons using EPHYS data collected via a microelectrode array (MEA). This system was later applied to human glutamatergic (Glu) neurons for eight days preceding a suborbital flight. A live-dead assay confirmed cell viability, and the system was integrated into a CubeLab to maintain a controlled environment. Two biological samples were flown, along with two control samples, to validate the EPHYS system. Results showed that human Glu-neurons exposed to microgravity exhibited altered expression of vesicular glutamate transporters (VGLUTs) while maintaining neuronal differentiation markers. The findings contribute to understanding neurological disorders, neuro-inflammation, and cognitive impacts of space travel, with broader applications for brain health research on Earth.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
npj Microgravity
npj Microgravity Physics and Astronomy-Physics and Astronomy (miscellaneous)
CiteScore
7.30
自引率
7.80%
发文量
50
审稿时长
9 weeks
期刊介绍: A new open access, online-only, multidisciplinary research journal, npj Microgravity is dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信