从空气到洞察:空气DNA测序技术的演变。

IF 2.6 4区 生物学 Q3 MICROBIOLOGY
Mia F G Berelson, Darren Heavens, Paul Nicholson, Matthew D Clark, Richard M Leggett
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引用次数: 0

摘要

从历史上看,空气中生物有机体的分析依赖于显微镜和基于培养的技术。然而,PCR和下一代测序等技术的进步现在为研究人员提供了收集大量空气环境DNA (eDNA)数据的能力。研究通常包括捕获空气中的生物材料,然后进行核酸提取、文库制备、测序和分类鉴定,以表征给定位置的eDNA。这些方法有多种应用,包括农业和人类健康中的病原体检测、空气质量监测、生物恐怖主义检测和生物多样性监测。航空eDNA分析方法多种多样,没有一种方法能满足所有需求。本综述概述了目前的采样、提取、测序和生物信息学分析方法,强调了不同的方法如何影响所得数据及其对特定用例的适用性。它还探讨了机载eDNA采样的当前应用,并确定了该领域的研究空白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
From air to insight: the evolution of airborne DNA sequencing technologies.

Historically, the analysis of airborne biological organisms relied on microscopy and culture-based techniques. However, technological advances such as PCR and next-generation sequencing now provide researchers with the ability to gather vast amounts of data on airborne environmental DNA (eDNA). Studies typically involve capturing airborne biological material, followed by nucleic acid extraction, library preparation, sequencing and taxonomic identification to characterize the eDNA at a given location. These methods have diverse applications, including pathogen detection in agriculture and human health, air quality monitoring, bioterrorism detection and biodiversity monitoring. A variety of methods are used for airborne eDNA analysis, as no single pipeline meets all needs. This review outlines current methods for sampling, extraction, sequencing and bioinformatic analysis, highlighting how different approaches can influence the resulting data and their suitability for specific use cases. It also explores current applications of airborne eDNA sampling and identifies research gaps in the field.

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来源期刊
Microbiology-Sgm
Microbiology-Sgm 生物-微生物学
CiteScore
4.60
自引率
7.10%
发文量
132
审稿时长
3.0 months
期刊介绍: We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms. Topics include but are not limited to: Antimicrobials and antimicrobial resistance Bacteriology and parasitology Biochemistry and biophysics Biofilms and biological systems Biotechnology and bioremediation Cell biology and signalling Chemical biology Cross-disciplinary work Ecology and environmental microbiology Food microbiology Genetics Host–microbe interactions Microbial methods and techniques Microscopy and imaging Omics, including genomics, proteomics and metabolomics Physiology and metabolism Systems biology and synthetic biology The microbiome.
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