前列腺癌患者细胞因子表达谱的比较分析。

IF 3.6 3区 生物学 Q1 BIOLOGY
Karoline Brito Caetano Andrade Coelho, Denise Kusma Wosniaki, Jonatas Luiz Pereira, Murilo Luz, Letusa Albrecht, Jeanine Marie Nardin, Mateus Nobrega Aoki, Leonardo O Reis, Rodolfo Borges Dos Reis, Dalila Lucíola Zanette
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引用次数: 0

摘要

本研究旨在鉴别前列腺癌(PCa)患者与健康人的细胞因子表达谱。采用Multiplex ELISA (Luminex)检测75例PCa患者和14例健康对照血浆中IL-4、IL-5、IL-6、IL-10、IL-1β、IL-17A、IL-12p70、MCP-1/CCL2、MIP-1α/CCL3、MIP-1β/CCL4、TNF-α和IFN-γ等12种细胞因子的表达水平。使用Mann-Whitney检验、Wilcoxon秩和检验、Spearman秩相关和K-means聚类无监督机器学习来验证细胞因子表达模式,分析细胞因子表达水平的差异。在PCa患者中,MIP-1α/CCL3、MIP-1β/CCL4、IFN-γ和白细胞介素的表达水平显著升高;相反,与健康个体相比,TNF-α和MCP-1/CCL2的表达均降低。聚类分析证实,PCa患者与MIP-1α/CCL3、IFN- γ、IL-12p70、IL-4和IL-5的相关性最高。此外,前列腺癌患者中IL-4与MIP-1 β、IL-4与ifn - γ、IL-5与IL-12p70、IL-5与ifn - γ之间的特异性相关性在健康个体中没有出现。这些结果将指导即将到来的体外和体内人类前列腺癌药物治疗模型,为探索未来的药物靶点和候选药物铺平道路,这些药物有可能通过靶向细胞因子水平和肿瘤发生途径来预测fda批准的前列腺癌治疗反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Analysis of Cytokine Expression Profiles in Prostate Cancer Patients.

This study aimed to identify the cytokine expression profile in prostate cancer (PCa) patients compared to healthy individuals. Plasma samples from 75 PCa patients and 14 healthy controls were analyzed using Multiplex ELISA (Luminex) to measure the expression levels of 12 cytokines: IL-4, IL-5, IL-6, IL-10, IL-1β, IL-17A, IL-12p70, MCP-1/CCL2, MIP-1α/CCL3, MIP-1β/CCL4, TNF-α, and IFN-γ. Differences in cytokine expression levels were analyzed using the Mann-Whitney test, Wilcoxon's rank-sum test, Spearman's rank correlation, and K-means Clustering unsupervised machine learning to validate cytokine expression patterns. In PCa patients, MIP-1α/CCL3, MIP-1β/CCL4, IFN-γ, and interleukins exhibited significantly higher expression levels; conversely, TNF-α and MCP-1/CCL2 both had decreased expression compared to healthy individuals. The clustering analysis confirmed that PCa patients exclusively exhibit the highest associations with MIP-1α/CCL3, IFN- γ, IL-12p70, IL-4, and IL-5. Furthermore, specific correlations between IL-4 and MIP-1 beta, IL-4 and IFN-gamma, IL-5 and IL-12p70, and IL-5 and IFN-gamma in PCa patients did not occur in healthy individuals. Such results will guide forthcoming in vitro and in vivo human prostate cancer-drug treatment models, paving the way for exploration of future drug targets and candidates with potential to predict FDA-approved prostate cancer treatment responses by targeting cytokine levels and the oncogenesis pathways.

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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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