Arthrospira platensis提取物对皮肤致病菌和皮肤癌细胞的抑制作用。

IF 3.6 3区 生物学 Q1 BIOLOGY
Ranchana Rungjiraphirat, Nitsanat Cheepchirasuk, Sureeporn Suriyaprom, Yingmanee Tragoolpua
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引用次数: 0

摘要

Arthrospira platensis(螺旋藻)是一种含有多种植物化学化合物的蓝藻,具有各种抗氧化、抗菌、抗病毒、抗癌、抗炎和免疫促进特性。本研究测定了白刺乙醇和甲醇粗提物的抑菌、抗氧化和抗癌作用。DPPH、ABTS、FRAP测定乙醇提取物的抗氧化能力最高,分别为8.96±0.84 mg没食子酸当量(mg GAE/g提取物)、53.03±4.21 mg trolox当量(mg TEAC/g提取物)和48.06±0.78 mg TEAC/g提取物。乙醇提取物中总酚和类黄酮含量最高,分别为38.79±1.61 mg GAE/g和27.50±0.53 mg槲皮素当量(mg QE/g提取物)。HPLC法测定了提取物中没食子酸和槲皮素的含量。采用琼脂孔扩散法和肉汤稀释法对金黄色葡萄球菌、表皮葡萄球菌、耐甲氧西林金黄色葡萄球菌(MRSA)、黄体微球菌、铜绿假单胞菌和痤疮角质杆菌等皮肤病原菌进行抑菌活性评价。白刺提取物的抑菌带范围为9.67±0.58 ~ 12.50±0.50 mm。MIC和MBC值在31.25 ~ 125 mg/mL之间。结晶紫染色法测定,白刺乙醇提取物对生物膜形成和根除的抑制作用分别为87.18%和99.77%。此外,我们还检测了platensis对A375人黑色素瘤细胞的抗癌活性。乙醇和甲醇提取物通过上调caspase-3、caspase-8和caspase-9的表达,通过内源性和外源性途径诱导DNA凋亡。这些研究结果表明,platensis具有良好的抗氧化、抗菌和抗癌活性,强调了其作为治疗病理疾病的天然药物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibitory Efficacy of Arthrospira platensis Extracts on Skin Pathogenic Bacteria and Skin Cancer Cells.

Arthrospira platensis (spirulina) is a cyanobacterium containing various phytochemical compounds associated with various antioxidant, antimicrobial, antiviral, anticancer, anti-inflammatory, and immune-promoting properties. The efficacy of ethanolic and methanolic crude extracts of A. platensis regarding antibacterial, antioxidant, and anticancer effects was determined in this study. The ethanolic extract showed the highest antioxidant activity by 8.96 ± 0.84 mg gallic acid equivalent per gram of extract (mg GAE/g extract), 53.03 ± 4.21 mg trolox equivalent antioxidant capacity per gram of extract (mg TEAC/g extract), and 48.06 ± 0.78 mg TEAC/g extract as determined by DPPH, ABTS, FRAP assays, respectively. Moreover, the ethanolic extract showed the highest total phenolic and flavonoid compound contents by 38.79 ± 1.61 mg GAE/g extract and 27.50 ± 0.53 mg of quercetin equivalent per gram of extract (mg QE/g extract). Gallic acid and quercetin in the extracts were also determined by HPLC. The antibacterial activity was evaluated by agar well diffusion and broth dilution methods on skin pathogenic bacteria, including Staphylococcus aureus, Staphylococcus epidermidis, methicillin-resistant S. aureus (MRSA), Micrococcus luteus, Pseudomonas aeruginosa, and Cutibacterium acnes. The inhibition zone of A. platensis extracts ranges from 9.67 ± 0.58 to 12.50 ± 0.50 mm. In addition, MIC and MBC values ranged from 31.25 to 125 mg/mL. The inhibition of biofilm formation and biofilm eradication by A. platensis ethanolic extract was 87.18% and 99.77%, as determined by the crystal violet staining assay. Furthermore, the anticancer activity of A. platensis on A375 human melanoma cells was examined. The ethanolic and methanolic extracts induced DNA apoptosis through both intrinsic and extrinsic pathways by upregulating the expression of caspase-3, caspase-8, and caspase-9. These findings suggested that A. platensis demonstrated promising antioxidant, antibacterial, and anticancer activities, emphasizing its potential as a natural therapeutic agent for the management of pathological conditions.

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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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