使用四种商业酶联免疫吸附试验和中和试验检测和比较定期大规模接种猪繁殖与呼吸综合征修饰活病毒的母猪血清样本

IF 2 2区 农林科学 Q2 VETERINARY SCIENCES
Chaosi Li, Gang Wang, Zhicheng Liu, Shuhe Fang, Aihua Fan, Kai Chen, Jianfeng Zhang
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引用次数: 0

摘要

采用猪繁殖与呼吸综合征病毒(PRRSV)修饰活病毒(MLV)疫苗接种控制PRRSV。在中国,农场每4到6个月对母猪群进行随机抽样。他们使用酶联免疫吸附试验(ELISA)方法,通过跟踪阳性率或样本阳性比来监测牛群的免疫状态。然而,在实施大规模疫苗接种并稳定生产的猪场,ELISA抗体的阳性率有所下降,特别是在高胎次母猪中。这对目前的PRRSV免疫监测方法提出了相当大的挑战。目前尚不清楚这是否反映了疫苗对这些特殊情况的敏感性不足,或者母猪真的失去了免疫力。在这项研究中,从中国不同地区的四个农场(A-D)获得了233份样本。采用4种代表性ELISA试剂盒(2种针对核衣壳蛋白(N), 2种针对糖蛋白(GP))检测PRRS免疫状态。A-D的阳性率分别为57.1-100%、50.9-100%、50-100%和75.7-100%。4种ELISA试剂盒的阳性率分别为50.0% ~ 75.7%、70.0 ~ 75.7%、82.5 ~ 97.1%和100%,一致性较差。胎次1和胎次2母猪的阳性率和体液抗体水平显著低于胎次高的母猪(bbbb4)。88份用ELISA试剂盒A鉴定的ELISA阴性样本用病毒中和试验(VNT)进行验证,只有15.9%的样本检测为阴性。总之,ELISA抗体阴性问题存在,主要发生在使用特定试剂盒检测的特定农场。然而,与VNT结果的低相关性和试剂盒之间的不一致性表明,依靠一种ELISA检测不足以监测接种PRRSV mlv的猪群的免疫状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection and Comparison of Sow Serum Samples from Herds Regularly Mass Vaccinated with Porcine Reproductive and Respiratory Syndrome Modified Live Virus Using Four Commercial Enzyme-Linked Immunosorbent Assays and Neutralizing Tests.

Porcine reproductive and respiratory syndrome virus (PRRSV) modified live virus (MLV) vaccination is used to control PRRSV. In China, farms conduct random sampling from sow herds every 4 to 6 months. They use the enzyme-linked immunosorbent assay (ELISA) method to monitor the immune status of the herd by tracking the positive rate or the sample-to-positive ratio. However, in farms that implement mass vaccination and have stable production, the positive rate of ELISA antibodies has decreased, especially in high-parity sows. This poses a considerable challenge to the current monitoring approach of PRRSV immunity. It remains unclear whether this reflects insufficient sensitivity of the kits for these special scenarios or the fact that the sows have truly lost immunity. In this study, 233 samples from four farms (A-D) across different regions of China were acquired. They were tested using four representative ELISA kits, two targeting the nucleocapsid protein (N) and two targeting the glycoprotein (GP) to evaluate PRRS immune status. The respective sample positive rates in A-D were 57.1-100%, 50.9-100%, 50-100%, and 75.7-100% using the kits. The positive rates using the four ELISA kits were 50.0-75.7%, 70.0-75.7%, 82.5-97.1%, and 100%, respectively, with poor agreement among them. The positive rates and humoral antibody levels for parity 1 and 2 sows were significantly lower than those with higher parities (>4). Eighty-eight ELISA-negative samples identified using ELISA kit A were verified using a viral neutralizing test (VNT), with only 15.9% of the samples testing negative. In conclusion, the ELISA antibody negativity issue existed, mostly occurring in specific farms tested using a specific kit. However, the low correlation with the VNT results and the poor agreements among the kits suggest that relying on one ELISA test is insufficient to monitor the immune status of PRRSV MLV-vaccinated herds.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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