KIF2C缺失导致小鼠减数分裂异常和非阻塞性无精子症。

IF 2.7 3区 医学 Q2 OBSTETRICS & GYNECOLOGY
Reproductive Medicine and Biology Pub Date : 2025-05-27 eCollection Date: 2025-01-01 DOI:10.1002/rmb2.12659
Hiroaki Kitakaze, Haruhiko Miyata, Yuki Oyama, Chen Pan, Yuma Kujime, Go Tsujimura, Takahiro Imanaka, Sohei Kuribayashi, Norichika Ueda, Kentaro Takezawa, Shinichiro Fukuhara, Norio Nonomura, Masahito Ikawa
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引用次数: 0

摘要

目的:激酶蛋白家族成员2C (KIF2C)是有丝分裂中微管动力学和染色体分离的关键调节因子。然而,它在精子发生中的作用仍不清楚。最近的转录组学分析表明,KIF2C与男性不育之间存在潜在联系。本研究旨在利用KIF2C敲除(KO)小鼠阐明KIF2C在精子发生中的作用。方法:为了克服与Kif2c缺失相关的断奶前致死率,我们培育了具有129X1/SvJ和B6D2混合遗传背景的Kif2c KO小鼠。我们评估了Kif2c KO小鼠的雄性生育能力、附睾精子计数和睾丸切片。结果:获得了全球性的Kif2c KO小鼠,并表现出雄性不育。组织学分析和附睾精子计数显示,Kif2c KO小鼠的精子发生严重受损,缺乏成熟精子。这些发现与非阻塞性无精子症(NOA)患者的观察结果一致。我们对Kif2c KO精小管的分类表明,大多数生精细胞在早期阶段被抑制,特别是在减数分裂期间。结论:本研究提供了体内证据,证明KIF2C对小鼠精子发生和雄性生育能力至关重要。全球Kif2c KO小鼠的成功产生为NOA建立了动物模型,支持了生殖细胞发育和生殖健康的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
KIF2C Deletion Causes Meiotic Abnormalities and Nonobstructive Azoospermia in Mice.

Purpose: Kinesin Family Member 2C (KIF2C) is a key regulator of microtubule dynamics and chromosome segregation in mitosis. However, its role in spermatogenesis remains unclear. Recent transcriptomic analyses suggest a potential link between KIF2C and male infertility. This study aimed to clarify KIF2C's roles in spermatogenesis using Kif2c knockout (KO) mice.

Methods: To overcome the preweaning lethality associated with Kif2c deletion, we generated Kif2c KO mice with a mixed genetic background of 129X1/SvJ and B6D2. We assessed male fertility, epididymal sperm counts, and testicular sections of Kif2c KO mice.

Results: Global Kif2c KO mice were obtained and showed male infertility. Histological analyses and epididymal sperm count revealed that Kif2c KO mice exhibited severely impaired spermatogenesis and absence of mature spermatozoa. These findings are consistent with those observed in patients with nonobstructive azoospermia (NOA). Our classification of Kif2c KO seminiferous tubules indicated that most spermatogenic cells were arrested at the early stages, particularly during meiosis.

Conclusions: This study provides in vivo evidence that KIF2C is essential for spermatogenesis and male fertility in mice. The successful generation of global Kif2c KO mice establishes an animal model for NOA, supporting research on germ cell development and reproductive health.

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来源期刊
CiteScore
5.70
自引率
5.90%
发文量
53
审稿时长
20 weeks
期刊介绍: Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.
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