首次从分子上证实摩洛哥长尾河马(双翅目:河马科)的存在和庇护犬的感染。

IF 3 2区 医学 Q1 PARASITOLOGY
Maria Bourquia, Abderrahmane Zahri, Mehdi Ahlamine, Thomas Balenghien, Paula Meyer, Felix Gregor Sauer, Renke Lühken
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引用次数: 0

摘要

背景:长尾河马(双翅目:河马科)是一种专性食血体外寄生虫,在非洲、南欧、中东和亚洲广泛感染脊椎动物宿主。它是龙尾丝虫病(丝虫病总纲:盘尾丝虫科)的潜在媒介,也是雅斯古利车蜱(车蜱螨科)的宿主,后者是一种已知的狗和人皮炎病原体。由于摩洛哥缺乏关于河马的数据,本研究旨在调查该国庇护犬的虱蝇动物群以及它们可能携带的丝状线虫。方法:在2022年4月至11月期间,随机调查了摩洛哥中部4个城市230只收容犬,作为节肢动物媒介和犬媒介传播病原体昆虫学和流行病学研究的一部分。随机收集家犬身上所有可见的蝇类,进行形态和分子鉴定。随后提取DNA进行丝状线虫的筛选。结果:30只犬(13.1%)共被长尾白僵虱35只,其中成虫33只(雄10只,母未孕19只,母孕4只),幼虫2只;通过细胞色素氧化酶I亚基基因的DNA条形码确认了两个具有代表性的标本。所有蝇池(妊娠雌蝇、未妊娠雌蝇、雄蝇和幼虫)的12S rRNA PCR检测均为丝状线虫阴性。结论:本研究首次对摩洛哥长尾长臂猿蝇类进行了形态和分子鉴定。需要进一步开展全国范围的调查,以解决对未记录的海马物种及其可能携带的具有医学和兽医重要性的病原体的知识差距。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
First molecular confirmation of the presence of Hippobosca longipennis (Diptera: Hippoboscidae) and infestation of sheltered dogs in Morocco.

Background: Hippobosca longipennis (Diptera: Hippoboscidae) is an obligate hematophagous ectoparasite that infests a wide range of vertebrate hosts across Africa, Southern Europe, the Middle East, and Asia. It is a potential vector of Acanthocheilonema dracunculoides (Filarioidea: Onchocercidae) and serves as a phoretic host for Cheyletiella yasguri (Acari: Cheyletiellidae), a known causative agent of dermatitis in both dogs and humans. Due to the lack of data on hippoboscids in Morocco, this study aimed to investigate the louse fly fauna of sheltered dogs in the country as well as the filarial nematodes they may harbor.

Methods: Between April and November 2022, 230 sheltered dogs from four cities in Central Morocco were randomly examined as part of an entomological and epidemiological study on arthropod vectors and canine vector-borne pathogens. All visible louse flies on the domestic dogs were randomly collected and then morphologically and molecularly identified. DNA was subsequently extracted for screening of filarial nematodes.

Results: A total of 30 dogs (13.1%) were infested with 35 H. longipennis louse flies, consisting of 33 adults (10 males, 19 non-gravid females, and four gravid females) and two larvae. Two representative specimens were confirmed through DNA barcoding of the cytochrome oxidase subunit I gene. All fly pools (gravid females, non-gravid females, males, and larvae) tested negative for filarial nematodes in the 12S rRNA PCR.

Conclusions: This study represents the first morphological and molecular characterization of H. longipennis flies in Morocco. Further national-scale investigations are needed to address gaps in the knowledge of unrecorded hippoboscid species and the pathogens of medical and veterinary importance that they may carry.

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来源期刊
Parasites & Vectors
Parasites & Vectors 医学-寄生虫学
CiteScore
6.30
自引率
9.40%
发文量
433
审稿时长
1.4 months
期刊介绍: Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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