Rene Nieves-Morales, Jessica Alejandra Paez-Diaz, Sofia Marie Rodriguez-Carrio, Gabriela Melendez Martinez, Edwin Omar Rivera-Lopez, Josué Rodríguez-Ramos, José E García-Arrarás, Carlos Rios-Velazquez
{"title":"利用ITS和16S rDNA扩增子从直接机械、酶和化学宏基因组提取褐岩海参（Holothuria glaberrima）肠道微生物组图谱。","authors":"Rene Nieves-Morales, Jessica Alejandra Paez-Diaz, Sofia Marie Rodriguez-Carrio, Gabriela Melendez Martinez, Edwin Omar Rivera-Lopez, Josué Rodríguez-Ramos, José E García-Arrarás, Carlos Rios-Velazquez","doi":"10.1128/mra.00293-25","DOIUrl":null,"url":null,"abstract":"<p><p>Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate <i>Holothuria glaberrima</i> was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber's intestinal biology.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0029325"},"PeriodicalIF":0.7000,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Intestinal microbiome profile of the brown rock sea cucumber (<i>Holothuria glaberrima</i>) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction.\",\"authors\":\"Rene Nieves-Morales, Jessica Alejandra Paez-Diaz, Sofia Marie Rodriguez-Carrio, Gabriela Melendez Martinez, Edwin Omar Rivera-Lopez, Josué Rodríguez-Ramos, José E García-Arrarás, Carlos Rios-Velazquez\",\"doi\":\"10.1128/mra.00293-25\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate <i>Holothuria glaberrima</i> was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber's intestinal biology.</p>\",\"PeriodicalId\":18654,\"journal\":{\"name\":\"Microbiology Resource Announcements\",\"volume\":\" \",\"pages\":\"e0029325\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2025-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology Resource Announcements\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1128/mra.00293-25\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology Resource Announcements","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1128/mra.00293-25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Intestinal microbiome profile of the brown rock sea cucumber (Holothuria glaberrima) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction.
Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate Holothuria glaberrima was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber's intestinal biology.
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