{"title":"结核性淋巴结炎的细胞形态学及其与抗酸杆菌巴氏法、Auramine-Rhodamine荧光和Ziehl-Neelsen技术检测的相关性。","authors":"Seethalakshmi Viswanathan, C. P. Shroff","doi":"10.1002/dc.25486","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Tuberculosis (TB) is a common cause of lymphadenopathy in developing countries. Fine needle aspiration cytology (FNAC) along with acid fast bacilli (AFB) detection is an invaluable diagnostic test. Fluorescent methods for AFB detection have greater sensitivity as compared to the Ziehl-Neelsen (ZN) method.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>The incidence of TB lymphadenitis was determined among all lymph nodes aspirated over 5 years, from January 1993 to May 1998 in an Indian referral hospital. Cytomorphological spectrum was studied in 234 smears, and AFB positivity was determined by Papanicolaou (PAP), Auramine-Rhodamine (AR) fluorescence as compared to the ZN method.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Of a total of 6592 lymph node aspirates, 33.86% were diagnosed as tuberculosis; maximum patients were between 11 and 30 years (68.5%), with a female predominance. Cervical nodes were commonly involved (85.6%). Tuberculosis was diagnosed in 41.4% of the 55 patients diagnosed with HIV infection. Cytomorphology in 234 smears examined predominantly showed caseous necrosis with degenerating histiocytes (48.7%). PAP method was the most sensitive for AFB detection (73.1%), followed by AR (57.7%) and ZN (42.3%) and the rate of AFB detection and bacillary load increased with the presence of caseous necrosis and polymorphonuclear cells and decreased with epithelioid cells, presence of caseous necrosis being statistically significant (<i>p</i> < 0.05).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Incidence of tuberculous lymphadenitis is high in developing countries. FNAC along with AFB detection is a reliable and definitive diagnostic procedure. Fluorescent techniques are more sensitive than ZN method for AFB detection, with certain limitations. Further studies may need to be done to evaluate their diagnostic utility over ZN method.</p>\n </section>\n </div>","PeriodicalId":11349,"journal":{"name":"Diagnostic Cytopathology","volume":"53 8","pages":"402-412"},"PeriodicalIF":1.0000,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dc.25486","citationCount":"0","resultStr":"{\"title\":\"Cytomorphology in Tuberculous Lymphadenitis and Correlation With Acid Fast Bacilli Detection by Papanicolaou, Auramine-Rhodamine Fluorescence and Ziehl-Neelsen Techniques\",\"authors\":\"Seethalakshmi Viswanathan, C. P. Shroff\",\"doi\":\"10.1002/dc.25486\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Tuberculosis (TB) is a common cause of lymphadenopathy in developing countries. Fine needle aspiration cytology (FNAC) along with acid fast bacilli (AFB) detection is an invaluable diagnostic test. Fluorescent methods for AFB detection have greater sensitivity as compared to the Ziehl-Neelsen (ZN) method.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>The incidence of TB lymphadenitis was determined among all lymph nodes aspirated over 5 years, from January 1993 to May 1998 in an Indian referral hospital. Cytomorphological spectrum was studied in 234 smears, and AFB positivity was determined by Papanicolaou (PAP), Auramine-Rhodamine (AR) fluorescence as compared to the ZN method.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Of a total of 6592 lymph node aspirates, 33.86% were diagnosed as tuberculosis; maximum patients were between 11 and 30 years (68.5%), with a female predominance. Cervical nodes were commonly involved (85.6%). Tuberculosis was diagnosed in 41.4% of the 55 patients diagnosed with HIV infection. Cytomorphology in 234 smears examined predominantly showed caseous necrosis with degenerating histiocytes (48.7%). PAP method was the most sensitive for AFB detection (73.1%), followed by AR (57.7%) and ZN (42.3%) and the rate of AFB detection and bacillary load increased with the presence of caseous necrosis and polymorphonuclear cells and decreased with epithelioid cells, presence of caseous necrosis being statistically significant (<i>p</i> < 0.05).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Incidence of tuberculous lymphadenitis is high in developing countries. FNAC along with AFB detection is a reliable and definitive diagnostic procedure. Fluorescent techniques are more sensitive than ZN method for AFB detection, with certain limitations. Further studies may need to be done to evaluate their diagnostic utility over ZN method.</p>\\n </section>\\n </div>\",\"PeriodicalId\":11349,\"journal\":{\"name\":\"Diagnostic Cytopathology\",\"volume\":\"53 8\",\"pages\":\"402-412\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dc.25486\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diagnostic Cytopathology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/dc.25486\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic Cytopathology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dc.25486","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Cytomorphology in Tuberculous Lymphadenitis and Correlation With Acid Fast Bacilli Detection by Papanicolaou, Auramine-Rhodamine Fluorescence and Ziehl-Neelsen Techniques
Background
Tuberculosis (TB) is a common cause of lymphadenopathy in developing countries. Fine needle aspiration cytology (FNAC) along with acid fast bacilli (AFB) detection is an invaluable diagnostic test. Fluorescent methods for AFB detection have greater sensitivity as compared to the Ziehl-Neelsen (ZN) method.
Methods
The incidence of TB lymphadenitis was determined among all lymph nodes aspirated over 5 years, from January 1993 to May 1998 in an Indian referral hospital. Cytomorphological spectrum was studied in 234 smears, and AFB positivity was determined by Papanicolaou (PAP), Auramine-Rhodamine (AR) fluorescence as compared to the ZN method.
Results
Of a total of 6592 lymph node aspirates, 33.86% were diagnosed as tuberculosis; maximum patients were between 11 and 30 years (68.5%), with a female predominance. Cervical nodes were commonly involved (85.6%). Tuberculosis was diagnosed in 41.4% of the 55 patients diagnosed with HIV infection. Cytomorphology in 234 smears examined predominantly showed caseous necrosis with degenerating histiocytes (48.7%). PAP method was the most sensitive for AFB detection (73.1%), followed by AR (57.7%) and ZN (42.3%) and the rate of AFB detection and bacillary load increased with the presence of caseous necrosis and polymorphonuclear cells and decreased with epithelioid cells, presence of caseous necrosis being statistically significant (p < 0.05).
Conclusion
Incidence of tuberculous lymphadenitis is high in developing countries. FNAC along with AFB detection is a reliable and definitive diagnostic procedure. Fluorescent techniques are more sensitive than ZN method for AFB detection, with certain limitations. Further studies may need to be done to evaluate their diagnostic utility over ZN method.
期刊介绍:
Diagnostic Cytopathology is intended to provide a forum for the exchange of information in the field of cytopathology, with special emphasis on the practical, clinical aspects of the discipline. The editors invite original scientific articles, as well as special review articles, feature articles, and letters to the editor, from laboratory professionals engaged in the practice of cytopathology. Manuscripts are accepted for publication on the basis of scientific merit, practical significance, and suitability for publication in a journal dedicated to this discipline. Original articles can be considered only with the understanding that they have never been published before and that they have not been submitted for simultaneous review to another publication.