lncRNA H19/Hmox1轴调控铁凋亡在蒽环类药物诱导的心脏毒性中的作用。

IF 2.1 4区 医学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Bayan Kadeerbieke, Li Wu, Yuan-Ming Zhang
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引用次数: 0

摘要

本研究探讨了蒽环类药物(ANT)诱导心脏毒性的分子机制,特别关注长链非编码RNA (lncRNA) H19/血红素加氧酶-1 (Hmox1)信号轴调控的铁死亡。回顾性分析了50例发生ant相关性心功能障碍的乳腺癌患者。临床评估包括左心室射血分数(LVEF)和血清标志物的测量,如心肌肌钙蛋白I (cTnI)、肌酸激酶mb (CK-MB)、n端前b型利钠肽(NT-proBNP)和血清铁水平。血清分析显示lncRNA H19显著下调,Hmox1显著上调,两者均与心功能受损和铁稳态破坏显著相关。为了进一步阐明其作用机制,我们建立了表柔比星(EPI)诱导的HL-1心肌细胞损伤模型。EPI暴露导致lncRNA H19的抑制,Hmox1的上调,诱导细胞凋亡和铁致细胞死亡。RNA-seq分析发现lncRNA H19通过Hmox1调节与铁代谢相关的潜在下游靶点。功能分析表明,lncRNA H19的过度表达减轻了epi诱导的铁凋亡,而Hmox1的过度表达逆转了这些保护作用。总之,这些发现确定了lncRNA H19/Hmox1轴在ant诱导的心脏毒性中是铁凋亡的关键调节因子,并表明它是减轻蒽环类化疗乳腺癌患者心脏损伤的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of lncRNA H19/Hmox1 axis regulating ferroptosis in anthracycline-induced cardiotoxicity.

This study investigates the molecular mechanisms underlying anthracyclines (ANT)-induced cardiotoxicity, with a specific focus on ferroptosis regulated by the long non-coding RNA (lncRNA) H19/heme oxygenase-1 (Hmox1) signaling axis. A retrospective analysis was performed on 50 breast cancer patients who developed ANT-associated cardiac dysfunction. Clinical assessments included measurements of left ventricular ejection fraction (LVEF) and serum markers, such as cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), N-terminal pro-B-type natriuretic peptide (NT-proBNP), and serum iron levels. Serum analysis revealed a marked downregulation of lncRNA H19 and upregulation of Hmox1, both significantly correlated with impaired cardiac function and disrupted iron homeostasis. To further elucidate the mechanism, an Epirubicin (EPI)-induced injury model in HL-1 cardiomyocytes was established. EPI exposure led to suppression of lncRNA H19, upregulation of Hmox1, and induction of apoptosis and ferroptotic cell death. RNA-seq analysis identified potential downstream targets linking lncRNA H19 to iron metabolism via Hmox1 modulation. Functional assays demonstrated that overexpression of lncRNA H19 mitigated EPI-induced ferroptosis, while enforced expression of Hmox1 reversed these protective effects. Collectively, these findings identify the lncRNA H19/Hmox1 axis as a critical regulator of ferroptosis in ANT-induced cardiotoxicity and suggest it as a potential therapeutic target for mitigating cardiac injury in breast cancer patients undergoing anthracycline chemotherapy.

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来源期刊
Drug and Chemical Toxicology
Drug and Chemical Toxicology 医学-毒理学
CiteScore
6.00
自引率
3.80%
发文量
99
审稿时长
3 months
期刊介绍: Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal. Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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