生地黄治疗血虚的机制：化学、代谢组学和肠道菌群的研究

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2025-04-17 DOI:10.1016/j.cjac.2025.100548

RuXi GAO, FanYi WANG, Xiang LIU, Chu YUAN, GuoShun SHAN

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引用次数: 0

摘要

目的通过分析生地黄炮制前后化学成分的变化及其对BDS小鼠代谢组学和肠道菌群的影响，探讨生地黄治疗BDS的作用机制。方法采用超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-TOF-MS）对地黄炮制前后的差异成分进行筛选。进行了药效学研究以验证治疗效果。此外，采用UPLC-Q-TOF-MS、顶空进样-气相色谱-质谱（HS-GC-MS）和16sRNA测序技术研究RRP对BDS小鼠代谢组学和肠道微生物群的调节作用。结果鉴定的主要鉴别成分为环烯醚萜类、苯乙醇苷类、糖类、苯甲醛类和5-羟甲基糠醛及其衍生物。RRP有效改善BDS小鼠的血液和能量代谢。通过调节不饱和脂肪酸的生物合成途径，增加厚壁菌门的丰度以提高丁酸水平，降低拟杆菌门的丰度以下调乙酸和丙酸水平，从而对BDS产生治疗作用。结论rrp通过调节代谢途径和肠道菌群平衡，减轻BDS小鼠的代谢状态，为其临床应用提供了科学依据。

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Mechanisms of radix rehmanniae praeparata in treating blood deficiency: A study on chemistry, metabolomics, and gut microbiota

Objective

To investigate the mechanism of Radix Rehmanniae Praeparata (RRP) in treating blood deficiency syndrome (BDS) by analyzing the changes in chemical components before and after processing, and its effects on metabolomics and gut microbiota in BDS mice.

Methods

Ultra-high-performance liquid chromatographyquadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was employed to screen differential components before and after the processing of Radix Rehmanniae (RR). Pharmacodynamic studies were conducted to validate the therapeutic effects. Additionally, UPLC-Q-TOF-MS, headspace sampling-gas chromatography-mass spectrometry (HS-GC-MS), and 16sRNA sequencing were used to investigate the regulatory effects of RRP on metabolomics and the gut microbiota in BDS mice.

Results

The primary differential components identified were iridoids, phenylethanoid glycosides, sugars, benzaldehydes, and 5-hydroxymethylfurfural and its derivatives. RRP effectively ameliorated blood and energy metabolism in BDS mice. It regulates the biosynthesis pathway of unsaturated fatty acids, increases the abundance of Firmicutes to elevate butyric acid levels, and reduces the abundance of Bacteroidetes to downregulate acetic and propionic acid levels, thereby exerting therapeutic effects on BDS.

Conclusion

RRP attenuated the metabolic state of BDS mice by modulating metabolic pathways and gut microbiota balance, providing a scientific basis for its clinical application.

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.