基于分支DNA纳米平台的mRNA疫苗在体内的有效免疫治疗

IF 13.2 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today Pub Date : 2025-05-27 DOI:10.1016/j.nantod.2025.102818

Changping Yang , Jing Fan , Hanyin Zhu , Huarui Liu , Xintong Li , Dandan Li , Hong Wang , Jianbing Liu , Baoquan Ding

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引用次数: 0

摘要

基于mRNA疫苗的免疫疗法已广泛应用于各种疾病的治疗。在此，我们报道了一种基于分支DNA纳米平台的mRNA疫苗，用于有效的体内免疫治疗。在我们的设计中，两个分支DNA结构被用作连接器，与涉及二硫键的RNA连接子共同组装。在这个支链DNA纳米平台上，具有5 ‘帽和3 ’聚A的mRNA可以通过RNA杂交高效装载。同时，通过DNA杂交，佐剂（CpG）和脂质（DOPE）可以被位点特异性包含。共组装后，表面带有脂质种子的纳米颗粒可作为脂质生长的模板。装载mrna和脂质包被的DNA纳米颗粒可以实现有效的细胞摄取和随后的刺激反应性药物释放，从而成功表达蛋白质。最后，肿瘤抗原编码的mRNA疫苗可以在体内引发明显的肿瘤抑制免疫治疗。这种合理开发的支链DNA纳米平台mRNA疫苗为免疫治疗的发展提供了一条新的途径。

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A branched DNA nanoplatform-based mRNA vaccine for efficient immunotherapy in vivo

Immunotherapy based on mRNA vaccine has been widely developed for treatments of various diseases. Herein, we report a branched DNA nanoplatform-based mRNA vaccine for efficient immunotherapy in vivo. In our design, two branched DNA structures are employed as the connectors to co-assemble with disulfide bonds-involved RNA linker. In this branched DNA nanoplatform, the mRNA with 5’ cap and 3’ poly A can be efficiently loaded by RNA hybridization. Meanwhile, the adjuvants (CpG) and lipids (DOPE) can be site-specifically included through DNA hybridization. After co-assembly, the nanoparticle with lipid seeds on the surface can function as a template for lipid growth. The mRNA-loaded and lipids-coated DNA nanoparticle can achieve an efficient cellular uptake and subsequent stimuli-responsive drug release for successful protein expression. Finally, the tumor antigen-encoded mRNA vaccine can elicit a pronounced immunotherapy for tumor inhibition in vivo. This rationally developed branched DNA nanoplatform-based mRNA vaccine presents a new avenue for the development of immunotherapy.

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来源期刊

Nano Today 工程技术-材料科学：综合

CiteScore

21.50

自引率

3.40%

发文量

305

审稿时长

40 days

期刊介绍： Nano Today is a journal dedicated to publishing influential and innovative work in the field of nanoscience and technology. It covers a wide range of subject areas including biomaterials, materials chemistry, materials science, chemistry, bioengineering, biochemistry, genetics and molecular biology, engineering, and nanotechnology. The journal considers articles that inform readers about the latest research, breakthroughs, and topical issues in these fields. It provides comprehensive coverage through a mixture of peer-reviewed articles, research news, and information on key developments. Nano Today is abstracted and indexed in Science Citation Index, Ei Compendex, Embase, Scopus, and INSPEC.