Neomycin Loaded by Tetrahedral Framework Nucleic Acids Enhances Antimicrobial Sensitivity against Bacteria

Burn wound infections pose significant challenges to burn injury management, and antibiotic therapy now is playing a crucial role in preventing and treating post-burn infections. Neomycin sulfate (NeoS), one of the most commonly used antibiotics for treating multiple bacterial infections, faces limitations such as low bioavailability and severe side effects. Therefore, there is an urgent need for strategies to improve the therapeutic efficacy of NeoS. This study proposes a strategy combining NeoS with nanomaterials, specifically using tetrahedral framework nucleic acids (tFNAs) as a carrier to load NeoS and fabricate tFNAs-loading NeoS (tFNAs-NeoS). This design made antibiotics more sensitive to Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), enabling reduced antibiotic dosages. Moreover, tFNA-NeoS exhibits improved stability, biocompatibility, and tissue utilization compared to free NeoS. Leveraging these advantages, tFNA-NeoS was tested in vivo using animal models, and the results further demonstrated its role in anti-inflammation activity, activating angiogenesis, and promoting wound healing. Thus, this strategy of using tFNAs to deliver antibiotics holds promise for enhancing antibiotic sensitivity and minimizing adverse effects in broader antibacterial scenarios.