一种双功能香豆素/菲菲啶融合探针，用于检测活细胞、拟南芥、斑马鱼和小鼠线粒体过氧亚硝酸盐

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-05-28 DOI:10.1016/j.snb.2025.138066

Xi Chen , Hui-Juan Lai , Wei-Na Wu , Xiao-Lei Zhao , Yun-Chang Fan , Lin-Yan Bian , Yuan Wang , Zhi-Hong Xu , Tony D. James

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引用次数: 0

摘要

异常过氧亚硝酸盐（ONOO−）可损伤多种生物分子，导致与多种氧化应激相关疾病密切相关的细胞功能障碍。因此，检测生理和病理过程中的ONOO−水平具有重要意义。本研究在DMSO/HEPES （v/v, 4/6, pH=7.4）溶液中，以高收率合成了香豆素/菲菲啶融合探针1，该探针对ONOO -具有高的荧光和紫外-可见选择性。探针在710 nm处有近红外（NIR）发射，Stokes位移为200 nm，通过添加ONOO−选择性猝灭。该探针用于跟踪活细胞、拟南芥、斑马鱼和小鼠中的ONOO−。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A bifunctional coumarin/phenanthridine-fused probe for the detection of mitochondrial peroxynitrite in live cells, Arabidopsis thaliana, zebrafish, and mice

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A bifunctional coumarin/phenanthridine-fused probe for the detection of mitochondrial peroxynitrite in live cells, Arabidopsis thaliana, zebrafish, and mice

Aberrant peroxynitrite (ONOO⁻) may damage a wide array of biomolecules, causing cellular dysfunctions that are closely associated with multiple oxidative stress-related diseases. Therefore, detecting ONOO⁻ levels in physiological and pathological processes is of great significance. With this research, a coumarin/phenanthridine-fused probe 1 was synthesized in high yield, and exhibited high selectivity toward ONOO⁻ for both fluorescence and UV–vis channels in DMSO/HEPES (v/v, 4/6, pH=7.4) solution. The probe exhibited a near-infrared (NIR) emission at 710 nm with a large Stokes shift of 200 nm, which was selectively quenched by adding ONOO⁻. The probe was used to track ONOO⁻ in live cells, Arabidopsis thaliana, zebrafish, and mice.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.