Monitoring the Biodegradation Progress of Naphthenic Acids in the Presence of Spirulina platensis Algae.

The experiments in this study investigated the toxicity of naphthenic acids (NAs) on the algal culture Spirulina platensis. The tests monitored the progression of the algal suspension in media contaminated with various concentrations of naphthenic acids. The evolution of the algal culture during the metabolism of NAs was investigated. The monitoring also included the determination of the values of some parameters during the biodegradation process (pH, conductivity, cell viability, dissolved oxygen). Optical density measurements (OD₆₀₀) were used to quantify the growth of Spirulina platensis, alongside the determination of the sedimentation index (IS). Cell viability was assessed microscopically using TEM and optical microscopy. The results facilitated the estimation of the percentage of cell growth inhibition and the inhibitory concentration value, determined by estimating ECb50 (concentration of NAs corresponding to 50% inhibition). The chemical quantification of naphthenic acids in the samples analyzed was performed by calculating the acidity value (AV) and characterizing the naphthenic acids through FTIR analysis. The graphical representation of ECb50 was established by extrapolating to a concentration of 110 mg/mL of naphthenic acids. We have demonstrated that pollution caused by NAs can be mitigated by the algae Spirulina platensis, which can metabolize these compounds and thus biodegrade them.