非诺沙丙- p-乙基除草剂诱导大鼠肾脏和睾丸生化、组织病理学和免疫组织化学改变。

IF 2.8 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2025-05-24 DOI:10.1016/j.reprotox.2025.108953

Aml I. Barakat , Ali Mohamed Eldib , Atef MK Nassar , Mohamed S.A. El-Gerbed , Nada S. Badr

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引用次数: 0

摘要

背景：农药的使用是农业中的一种全球性做法，它造成环境污染并构成重大健康风险。本研究探讨了手性除草剂Fenoxaprop-P-ethyl （FPE）对白化大鼠肾脏功能和雄性生殖能力的影响。材料和方法：该研究对8周龄雄性白化大鼠进行了实验，实验方案如下：对照组，载具组和两个fpe处理组：一个给予26mg/kg，另一个给予157.5mg/kg (1/20 LD₅0)30天。采集血样进行生化分析，对肾脏和睾丸组织进行氧化应激标志物、彗星测定、组织病理学检查和免疫组织化学分析。结果：研究表明，fpe处理的大鼠肾脏生物标志物呈剂量依赖性增加，同时出现氧化还原失衡和DNA损伤，肾脏组织改变，炎症细胞因子肿瘤坏死因子α和纤维化标志物转化生长因子β 1的表达增加。此外，fpe处理的大鼠性激素减少，氧化还原失衡，DNA损伤，睾丸生精层损伤，增殖细胞核抗原表达降低。结论：亚慢性暴露于FPE可导致肾脏损伤、炎症和纤维化，并伴有男性生殖健康的破坏。这项研究强调了氧化应激、激素失衡和生殖功能障碍之间复杂的关系。数据和材料的可获得性：“不适用”。

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Fenoxaprop-P-ethyl herbicide induces biochemical, histopathological, and immunohistochemical alterations in the kidneys and testes of rats

Background

Pesticide use, a global practice in agriculture, contributes to environmental contamination and poses significant health risks. This study investigates the impact of Fenoxaprop-P-ethyl (FPE), a chiral herbicide, on renal function and male fertility in albino rats.

Materials and methods

The study was conducted on 8-week-old male albino rats subjected to an experimental protocol as follows: the control group, a vehicle group, and two FPE-treated groups: one given 26 mg/kg and the other 157.5 mg/kg (1/20 LD₅₀) for 30 days. Blood samples were collected for biochemical analysis, and oxidative stress markers, comet assay assessment, histopathological examination, and immunohistochemical analysis were performed on renal and testicular tissues.

Results

The study shows that FPE-treated rats experience a dose-dependent increase in renal biomarkers, along with redox imbalance, and DNA damage, with kidney tissue alterations and increased expression of inflammatory cytokine tumor necrosis factor-alpha and fibrogenic marker transforming growth factor beta 1. Additionally, FPE-treated rats experience reduced sex hormones, along with redox imbalance, DNA damage, with testicular spermatogenic layer damage, and decreased proliferating cell nuclear antigen expression.

Conclusion

Sub-chronic exposure to FPE induces renal damage, inflammation, and fibrosis, accompanied by disruptions in male reproductive health. The study emphasizes the intricate relationship between oxidative stress, hormonal imbalance, and reproductive dysfunction.

Data Availability

Not applicable.

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.