Chartarlactams U-X：源自海洋真菌Stachybotrys sp. sfu - w23的新型苯螺烷，具有NF-κB/ROS信号通路介导的抗炎活性。

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2025-05-20 DOI:10.3390/md23050216

Yanhua Wu, Lanyi Lu, Peng Zhang, Liyan Wang

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引用次数: 0

摘要

本研究系统地探讨了海洋源真菌Stachybotrys sp. SZU-W23产生的苯螺驱烷（psd）的抗炎潜能。成功分离了15个psd。其中，4个新化合物被命名为chartarlactams U-X，通过NMR、hresms和ECD分析进行了精确的表征。具体来说，化合物10在0.3 ~ 30 μM浓度范围内对lps刺激的RAW 264.7巨噬细胞产生一氧化氮的抑制作用最强，IC50值为12.4 μM。此外，MTT试验显示在这些浓度下没有检测到细胞毒性。机制研究表明，化合物10有效抑制ROS的产生，可能使NF-κB信号通路失活，从而下调促炎介质，包括iNOS、IL-6和IL-1β。

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Chartarlactams U-X: Novel Phenylspirodrimanes from a Marine Derived Fungus Stachybotrys sp. SZU-W23 with Anti-Inflammatory Activity Mediated by the NF-κB/ROS Signaling Pathways.

In this investigation, the anti-inflammatory potential of phenylspirodrimanes (PSDs) produced by the marine-derived fungal strain Stachybotrys sp. SZU-W23 was systematically explored. A total of 15 PSDs were successfully isolated. Among them, four novel compounds, designated as chartarlactams U-X, were precisely characterized using NMR, HRESIMS, and ECD analyses. Specifically, compound 10 exhibited the most potent inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 macrophages within the 0.3-30 μM concentration range, with an IC₅₀ value of 12.4 μM. Additionally, MTT assays revealed no detectable cytotoxicity at these concentrations. Mechanistic studies revealed that compound 10 effectively suppressed ROS generation, likely inactivating the NF-κB signaling pathway and consequently downregulating pro-inflammatory mediators, including iNOS, IL-6, and IL-1β.

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.