{"title":"海水中水生Pontitalea aquvivens gen. nov., sp. nov.的多相分类分析。","authors":"Jaewoo Yoon","doi":"10.1007/s00284-025-04285-z","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-negative, rod-shaped, strictly aerobic, non-motile, and chemo-organoheterotrophic alphaproteobacterium, designated KMU-169<sup>T</sup>, was isolated from coastal seawater in the Republic of Korea. The novel isolate was able to grow at 0-2.0% NaCl concentrations (w/v), pH 6.0-9.5, and 15-40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-169<sup>T</sup> belongs to the family Paracoccaceae and shared the highest similarity (97.0%) with \"Defluviimonas salinarum\" CAU 1641<sup>T</sup>. The major (> 10%) cellular fatty acids were C<sub>18:1</sub> ω7c and summed feature 3 (comprising C<sub>16:1</sub> ω6c and/or C<sub>16:1</sub> ω7c). The predominant respiratory quinone was ubiquinone-10. Strain KMU-169<sup>T</sup> comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid, and an unidentified lipid. The assembled draft genome size of strain KMU-169<sup>T</sup> was 4.1 Mbp with a DNA G + C content of 65.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between the genomes of strain KMU-169<sup>T</sup> and its closely related taxa were 71.9-76.8%, 60.9-76.1%, and 18.4-21.0%, respectively. The genome of the strain KMU-169<sup>T</sup> revealed the presence of numerous genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially valuable enzymes. Based on the polyphasic taxonomic data reported in this study, a novel genus and a new species of the family Paracoccaceae, for which the name Pontitalea aquivivens gen. nov., sp. nov., is proposed with the type strain KMU-169<sup>T</sup> (= KCCM 90598<sup>T</sup> = NBRC 117093<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"308"},"PeriodicalIF":2.3000,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Polyphasic Taxonomic Analysis of Pontitalea aquivivens gen. nov., sp. nov., Isolated from Seawater.\",\"authors\":\"Jaewoo Yoon\",\"doi\":\"10.1007/s00284-025-04285-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A Gram-stain-negative, rod-shaped, strictly aerobic, non-motile, and chemo-organoheterotrophic alphaproteobacterium, designated KMU-169<sup>T</sup>, was isolated from coastal seawater in the Republic of Korea. The novel isolate was able to grow at 0-2.0% NaCl concentrations (w/v), pH 6.0-9.5, and 15-40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-169<sup>T</sup> belongs to the family Paracoccaceae and shared the highest similarity (97.0%) with \\\"Defluviimonas salinarum\\\" CAU 1641<sup>T</sup>. The major (> 10%) cellular fatty acids were C<sub>18:1</sub> ω7c and summed feature 3 (comprising C<sub>16:1</sub> ω6c and/or C<sub>16:1</sub> ω7c). The predominant respiratory quinone was ubiquinone-10. Strain KMU-169<sup>T</sup> comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid, and an unidentified lipid. The assembled draft genome size of strain KMU-169<sup>T</sup> was 4.1 Mbp with a DNA G + C content of 65.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between the genomes of strain KMU-169<sup>T</sup> and its closely related taxa were 71.9-76.8%, 60.9-76.1%, and 18.4-21.0%, respectively. The genome of the strain KMU-169<sup>T</sup> revealed the presence of numerous genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially valuable enzymes. Based on the polyphasic taxonomic data reported in this study, a novel genus and a new species of the family Paracoccaceae, for which the name Pontitalea aquivivens gen. nov., sp. nov., is proposed with the type strain KMU-169<sup>T</sup> (= KCCM 90598<sup>T</sup> = NBRC 117093<sup>T</sup>).</p>\",\"PeriodicalId\":11360,\"journal\":{\"name\":\"Current Microbiology\",\"volume\":\"82 7\",\"pages\":\"308\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00284-025-04285-z\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-025-04285-z","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Polyphasic Taxonomic Analysis of Pontitalea aquivivens gen. nov., sp. nov., Isolated from Seawater.
A Gram-stain-negative, rod-shaped, strictly aerobic, non-motile, and chemo-organoheterotrophic alphaproteobacterium, designated KMU-169T, was isolated from coastal seawater in the Republic of Korea. The novel isolate was able to grow at 0-2.0% NaCl concentrations (w/v), pH 6.0-9.5, and 15-40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-169T belongs to the family Paracoccaceae and shared the highest similarity (97.0%) with "Defluviimonas salinarum" CAU 1641T. The major (> 10%) cellular fatty acids were C18:1 ω7c and summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c). The predominant respiratory quinone was ubiquinone-10. Strain KMU-169T comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid, and an unidentified lipid. The assembled draft genome size of strain KMU-169T was 4.1 Mbp with a DNA G + C content of 65.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between the genomes of strain KMU-169T and its closely related taxa were 71.9-76.8%, 60.9-76.1%, and 18.4-21.0%, respectively. The genome of the strain KMU-169T revealed the presence of numerous genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially valuable enzymes. Based on the polyphasic taxonomic data reported in this study, a novel genus and a new species of the family Paracoccaceae, for which the name Pontitalea aquivivens gen. nov., sp. nov., is proposed with the type strain KMU-169T (= KCCM 90598T = NBRC 117093T).
期刊介绍：
Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment.
Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas:
physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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