双硫醇-稳定剂覆盖金纳米团簇的无共物和单电位通道电化学发光及其近红外免疫分析应用

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-21 DOI:10.1016/j.bios.2025.117617

Mengwei Li, Xuwen Gao, Xiaoxuan Ren, Luyao Ma, Yujiao Wang, Guizheng Zou

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引用次数: 0

摘要

Ru(bpy)32+/三丙胺（TPrA）是唯一的电化学发光（ECL）系统，在商业化体外诊断中得到广泛应用。Ru(bpy)32+/TPrA的共反应物ECL具有多通道发射和宽电化学电位窗口性质。本文提出了一种无共聚物、单电位通道的ECL策略，以生物相容性纳米电化学发光团簇，即二氢硫酸覆盖的Au纳米团簇（DHLA-AuNCs）为模型，其中DHLA的两个巯基和Au元素之间的金属-硫键使得DHLA-AuNCs具有富电子和n型性质的S空位。DHLA-AuNCs可被电化学氧化，外源空穴与内源自由电子之间的湮灭反应可发射出一种无共反应物的单电位通道ECL， ECL在+0.70 ~ + 1.20 V （vs Ag/AgCl）之间，最大发射电位为+0.91 V (vs Ag/AgCl)，最大发射波长为778 nm。ECL可方便地实现对人癌胚抗原的选择性检测，线性响应范围为20 ~ 5000 pg/mL，检出限为5 pg/mL。DHLA-AuNCs的无共反应物和单电位通道ECL为Ru(bpy)32+/TPrA的共反应物ECL提供了一种替代方案，以减少电化学干扰和信号串扰。

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Coreactant-free and single-potential-channel electrochemiluminescence from dual-thiols-stabilizer capped gold nanoclusters and its NIR immunoassay application

Ru(bpy)₃²⁺/tripropylamine (TPrA) is the sole electrochemiluminescence (ECL) system, which is being extensively employed in commercialized In Vitro Diagnosis. The coreactant ECL of Ru(bpy)₃²⁺/TPrA is of a multiple-channel emission and wide electrochemical potential window nature. Herein, a coreactant-free and single-potential-channel ECL strategy is proposed with a biocompatible nano-electrochemiluminophore, i.e., dihydrolipoic acid-capped Au nanoclusters (DHLA-AuNCs) as model, in which the metal-sulfur bonds between two sulfhydryls of DHLA and the Au element enable AuNCs with a S vacancies involved electron-rich and n-type nature for DHLA-AuNCs. The DHLA-AuNCs can be electrochemically oxidized, the annihilation reaction between exogenous holes and endogenous free-electrons can emit a kind of coreactant-free single-potential-channel ECL between +0.70 and + 1.20 V (vs Ag/AgCl) with the maximum emission potential of +0.91 V (vs Ag/AgCl) and the maximum emission wavelength of 778 nm. The ECL can be conveniently achieved, and selectively determine human carcinoembryonic antigen with the linear response range from 20 to 5000 pg/mL and the limit of detection of 5 pg/mL. The coreactant-free and single-potential-channel ECL of DHLA-AuNCs provides an alternative to the coreactant ECL of Ru(bpy)₃²⁺/TPrA for less electrochemical-interference and signal-crosstalk.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.