短通信:猪的印迹和父系表达的MIMT1 lncRNA同源性

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Jinsoo Ahn, In-Sul Hwang, Mi-Ryung Park, Seongsoo Hwang, In-Cheol Cho, Kichoon Lee
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引用次数: 0

摘要

哺乳动物基因的一个子集经历了基因组印记,印迹基因的亲本来源依赖表达是正常发育所必需的。尽管在猪身上已经记录了大约40个印迹基因,但需要对印迹结构域进行更详细的研究,以揭示未表征的猪长链非编码rna (lncRNAs)的印迹。我们的研究目的是研究同源MIMT1 lncRNA转录本在猪体内的印迹状态,并探索其在PEG3印迹结构域中的多种组织和条件下的等位基因表达。为此,我们比较了孤雌生殖和对照猪胚胎的单碱基分辨率DNA甲基组,并在PEG3和MIMT1基因共享的启动子区域发现了一个差异甲基化区域(DMR)。随后,在对照胚胎中,MIMT1 lncRNA转录物的表达显著增加(p <;0.05),表明其具有父系印记表达。基于单核苷酸变异,进一步分析表明,除了证实PEG3的父系单等位基因表达外,猪脑中也富集了MIMT1 lncRNA转录本,并在脑及其亚区进行单等位基因表达,表达的等位基因来自父系。本研究结果为进一步研究MIMT1印迹lncRNA在猪生长发育中的作用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Short Communication: The imprinted and paternally expressed MIMT1 lncRNA ortholog in pigs
A subset of mammalian genes undergoes genomic imprinting, and parent-of-origin-dependent expression of imprinted genes is required for normal development. Although around 40 imprinted genes have been documented in pigs, more detailed investigations into imprinted domains are needed to uncover the imprinting of uncharacterized porcine long non-coding RNAs (lncRNAs). Our study objectives were to investigate the imprinting status of orthologous MIMT1 lncRNA transcripts in pigs, which remains unidentified, and to explore their allelic expression in multiple tissues and conditions within the PEG3 imprinted domain. To this end, we compared the single-base resolution DNA methylome between parthenogenetic and control porcine embryos and identified a differentially methylated region (DMR) that is maternally methylated in the promoter region shared by PEG3 and MIMT1 genes. This was followed by a significantly higher expression of the MIMT1 lncRNA transcript in control embryos (p &lt; 0.05), suggesting its imprinted paternal expression. Based on single nucleotide variants, further analyses revealed that, in addition to confirming the paternal monoallelic expression of PEG3, MIMT1 lncRNA transcripts were enriched in the porcine brain and monoallelically expressed in the brain and its subregions, with the expressed alleles being paternally derived. Our results provide a foundation for investigating the role of the imprinted MIMT1 lncRNA in the growth and development of pigs.
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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