Jinsoo Ahn, In-Sul Hwang, Mi-Ryung Park, Seongsoo Hwang, In-Cheol Cho, Kichoon Lee
{"title":"短通信:猪的印迹和父系表达的MIMT1 lncRNA同源性","authors":"Jinsoo Ahn, In-Sul Hwang, Mi-Ryung Park, Seongsoo Hwang, In-Cheol Cho, Kichoon Lee","doi":"10.1093/jas/skaf182","DOIUrl":null,"url":null,"abstract":"A subset of mammalian genes undergoes genomic imprinting, and parent-of-origin-dependent expression of imprinted genes is required for normal development. Although around 40 imprinted genes have been documented in pigs, more detailed investigations into imprinted domains are needed to uncover the imprinting of uncharacterized porcine long non-coding RNAs (lncRNAs). Our study objectives were to investigate the imprinting status of orthologous MIMT1 lncRNA transcripts in pigs, which remains unidentified, and to explore their allelic expression in multiple tissues and conditions within the PEG3 imprinted domain. To this end, we compared the single-base resolution DNA methylome between parthenogenetic and control porcine embryos and identified a differentially methylated region (DMR) that is maternally methylated in the promoter region shared by PEG3 and MIMT1 genes. This was followed by a significantly higher expression of the MIMT1 lncRNA transcript in control embryos (p < 0.05), suggesting its imprinted paternal expression. Based on single nucleotide variants, further analyses revealed that, in addition to confirming the paternal monoallelic expression of PEG3, MIMT1 lncRNA transcripts were enriched in the porcine brain and monoallelically expressed in the brain and its subregions, with the expressed alleles being paternally derived. Our results provide a foundation for investigating the role of the imprinted MIMT1 lncRNA in the growth and development of pigs.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"51 1","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Short Communication: The imprinted and paternally expressed MIMT1 lncRNA ortholog in pigs\",\"authors\":\"Jinsoo Ahn, In-Sul Hwang, Mi-Ryung Park, Seongsoo Hwang, In-Cheol Cho, Kichoon Lee\",\"doi\":\"10.1093/jas/skaf182\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A subset of mammalian genes undergoes genomic imprinting, and parent-of-origin-dependent expression of imprinted genes is required for normal development. Although around 40 imprinted genes have been documented in pigs, more detailed investigations into imprinted domains are needed to uncover the imprinting of uncharacterized porcine long non-coding RNAs (lncRNAs). Our study objectives were to investigate the imprinting status of orthologous MIMT1 lncRNA transcripts in pigs, which remains unidentified, and to explore their allelic expression in multiple tissues and conditions within the PEG3 imprinted domain. To this end, we compared the single-base resolution DNA methylome between parthenogenetic and control porcine embryos and identified a differentially methylated region (DMR) that is maternally methylated in the promoter region shared by PEG3 and MIMT1 genes. This was followed by a significantly higher expression of the MIMT1 lncRNA transcript in control embryos (p < 0.05), suggesting its imprinted paternal expression. Based on single nucleotide variants, further analyses revealed that, in addition to confirming the paternal monoallelic expression of PEG3, MIMT1 lncRNA transcripts were enriched in the porcine brain and monoallelically expressed in the brain and its subregions, with the expressed alleles being paternally derived. Our results provide a foundation for investigating the role of the imprinted MIMT1 lncRNA in the growth and development of pigs.\",\"PeriodicalId\":14895,\"journal\":{\"name\":\"Journal of animal science\",\"volume\":\"51 1\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-05-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of animal science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1093/jas/skaf182\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of animal science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jas/skaf182","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Short Communication: The imprinted and paternally expressed MIMT1 lncRNA ortholog in pigs
A subset of mammalian genes undergoes genomic imprinting, and parent-of-origin-dependent expression of imprinted genes is required for normal development. Although around 40 imprinted genes have been documented in pigs, more detailed investigations into imprinted domains are needed to uncover the imprinting of uncharacterized porcine long non-coding RNAs (lncRNAs). Our study objectives were to investigate the imprinting status of orthologous MIMT1 lncRNA transcripts in pigs, which remains unidentified, and to explore their allelic expression in multiple tissues and conditions within the PEG3 imprinted domain. To this end, we compared the single-base resolution DNA methylome between parthenogenetic and control porcine embryos and identified a differentially methylated region (DMR) that is maternally methylated in the promoter region shared by PEG3 and MIMT1 genes. This was followed by a significantly higher expression of the MIMT1 lncRNA transcript in control embryos (p < 0.05), suggesting its imprinted paternal expression. Based on single nucleotide variants, further analyses revealed that, in addition to confirming the paternal monoallelic expression of PEG3, MIMT1 lncRNA transcripts were enriched in the porcine brain and monoallelically expressed in the brain and its subregions, with the expressed alleles being paternally derived. Our results provide a foundation for investigating the role of the imprinted MIMT1 lncRNA in the growth and development of pigs.
期刊介绍:
The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year.
Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.