仔猪体内胃肠道的消化参数和肠道菌群负荷及组成

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Chloë Rotsaert, Yorick Minnebo, Cindy Duysburgh, Lin Shu Liu, Karley K Mahalak, Jenni Firman, Lisa M Mattei, Ahmed M Moustafa, Kyle Bittinger, Weiming Hu, Massimo Marzorati, Joris Michiels, Tom Van de Wiele
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引用次数: 0

摘要

对肠道微生物群在动物和人类健康和疾病中的作用的日益关注,推动了对更相关和更准确的研究模型的需求。在本研究中,为了更好地了解不同胃肠段消化和发酵过程的体内动态,我们对10头TopigsNorsvin x German pi日新月异的仔猪的整个消化道的生化和微生物参数进行了概述,因为猪被认为是人类胃肠道的代表性动物模型。我们的主要发现包括区域特异性和显著差异(P <;其中胃pH值最低(3.36±0.72),回肠pH值最高(7.24±0.18)。干物质含量也有显著差异(P <;0.001),其中胃最高(27.8±2.4%),十二指肠最低(10.6±0.7%)。平均总穿越时间为12小时45分±1小时42分。酶活性(胃蛋白酶、胰蛋白酶、淀粉酶)存在个体间差异。仔猪氨基酸水平各不相同,十二指肠平均总浓度为7.04 × 102±2.29 × 102µg mL-1,空肠平均总浓度为1.19 × 103±2.69 × 102µg mL-1,回肠平均总浓度为9.39 × 102±2.54 × 102µg mL-1。不同仔猪的胆汁酸浓度差异很大,胆囊中的胆汁酸含量很高,而整个消化道的胆汁酸含量不同。短链脂肪酸浓度显著升高(P <;0.001),在大肠中含量最高。微生物负荷持续增加(P <;0.001),其中直肠的细胞负荷最高(6.82 × 1010±2.88 × 1010细胞mL-1)。微生物多样性最高的是下肠(即盲肠、结肠和直肠),微生物群落组成有显著的变化,特别是从回肠到盲肠。这项研究为猪肠道的消化和微生物参数提供了有价值的见解,证实了猪作为胃肠道研究模型的相关性。这些发现可以为体外或离体模型的开发提供信息,减少动物研究的伦理约束,并有助于评估饮食干预对肠道健康的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Digestive parameters and gut microbiota load and composition along the in vivo piglet gastrointestinal tract
The increased attention towards the role of the gut microbiome in health and disease for both animals and humans has fuelled the demand for more relevant and accurate research models. In this study, we present an overview of biochemical and microbial parameters measured throughout the digestive tract of ten TopigsNorsvin x German Piétrain piglets to better understand the in vivo dynamics of digestive and fermentative processes in different gastrointestinal segments, as pigs are suggested to be a representative animal model for the human gastrointestinal tract. Our key findings include region-specific and significantly differing (P &lt; 0.001) pH profiles, with the stomach having the lowest pH (3.36 ± 0.72) and the ileum the highest (7.24 ± 0.18). Dry matter content also varied significantly (P &lt; 0.001), with the stomach having the highest (27.8 ± 2.4%) and the duodenum the lowest (10.6 ± 0.7%). The average total transit time was 12 hours and 45 minutes ± 1 hour and 42 minutes. Enzyme activities (pepsin, trypsin, amylase) showed interindividual differences. Amino acid levels varied among piglets, with total concentrations averaging 7.04 x 102 ± 2.29 x 102 µg mL-1 in the duodenum, 1.19 x 103 ± 2.69 x 102 µg mL-1 in the jejunum and 9.39 x 102 ± 2.54 x 102 µg mL-1 in the ileum. Bile acid concentrations varied strongly between piglets, with high levels in the gall bladder and varying levels throughout the digestive tract. Short-chain fatty acid concentrations increased significantly (P &lt; 0.001) along the digestive tract, with the highest levels in the large intestine. The microbial load increased consistently (P &lt; 0.001) along the digestive tract, with the highest loads in the rectum (6.82 x 1010 ± 2.88 x 1010 cells mL-1). The highest microbial diversity was observed in the lower intestine (i.e. caecum, colon and rectum), with significant shifts in microbial community composition, especially from the ileum to the caecum. This study provides valuable insights into the digestive and microbiological parameters of the porcine gut, confirming the pig's relevance as a model for gastrointestinal research. The findings can inform the development of in vitro or ex vivo models, reducing ethical constraints of animal studies and aiding in the assessment of dietary interventions on gut health.
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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