细胞外囊泡相关表皮生长因子受体作为肺腺癌潜在的液体活检生物标志物:一项病例对照研究

IF 1.4 Q3 MEDICINE, GENERAL & INTERNAL
Journal of Yeungnam medical science Pub Date : 2025-01-01 Epub Date: 2025-05-23 DOI:10.12701/jyms.2025.42.36
Dian Jamel Salih
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引用次数: 0

摘要

背景:由于肺癌组织活检的局限性,细胞外囊泡(EVs)最近成为液体活检的潜在无创生物标志物。本研究探讨了ev相关表皮生长因子受体(EGFR)在肺腺癌中的存在。方法:分别从32例肺腺癌患者血清、32例健康对照、A549和BEAS-2B细胞系条件培养基中采集ev。采用超离心和隔离层析分离ev。通过透射电子显微镜、纳米颗粒跟踪分析(NTA)和免疫印迹(western blotting)证实了它们的特征。结果:NTA显示肺癌患者血清中EV浓度比健康对照组增加两倍。同样,A549细胞比BEAS-2B细胞分泌更多的ev。Western blotting验证了典型EV标记物的检测,如TSG101、CD81和flotillin-1,以及钙连联素的缺失。值得注意的是,从A549细胞和患者血清中分离出的EVs中,EGFR都被高度包装,而从健康对照和BEAS-2B细胞分离出的EVs中,EGFR很少存在或不存在。结论:我们的研究结果表明,EGFR被选择性地包装到来自肺腺癌的ev中,而在非癌对照中则不存在。ev相关EGFR可作为肺腺癌液体活检早期检测的无创指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Extracellular vesicle-associated epidermal growth factor receptor as a potential liquid biopsy biomarker in lung adenocarcinoma: a case-control study.

Extracellular vesicle-associated epidermal growth factor receptor as a potential liquid biopsy biomarker in lung adenocarcinoma: a case-control study.

Extracellular vesicle-associated epidermal growth factor receptor as a potential liquid biopsy biomarker in lung adenocarcinoma: a case-control study.

Extracellular vesicle-associated epidermal growth factor receptor as a potential liquid biopsy biomarker in lung adenocarcinoma: a case-control study.

Background: Extracellular vesicles (EVs) have recently emerged as potential noninvasive biomarkers for liquid biopsy because of the limitations of tissue biopsies in lung cancer. This study investigated the presence of EV-associated epidermal growth factor receptor (EGFR) in lung adenocarcinoma.

Methods: EVs were collected from the serum samples of 32 patients with lung adenocarcinoma, 32 healthy controls, and conditioned culture media from A549 and BEAS-2B cell lines. EVs were isolated using ultracentrifugation and size-exclusion chromatography. Their characteristic features were confirmed by transmission electron microscopy, nanoparticle tracking analysis (NTA), and western blotting.

Results: NTA revealed a two-fold increase in EV concentration in the serum of patients with lung cancer compared to healthy controls. Similarly, A549 cells secrete significantly more EVs than BEAS-2B cells. Western blotting validated the detection of canonical EV markers, such as TSG101, CD81, and flotillin-1, as well as the absence of calnexin. Notably, EGFR was highly packaged in the EVs isolated from both A549 cells and patient serum, whereas it was minimally present or absent in the EVs isolated from healthy controls and BEAS-2B cells.

Conclusion: Our findings indicated that EGFR was selectively packaged into EVs derived from lung adenocarcinoma and was absent in non-cancerous controls. EV-associated EGFR could be a noninvasive indicator for the early detection of lung adenocarcinoma through liquid biopsy.

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