Potential roles of the sirtuins in promoting longevity for larger Argopecten scallops.

Annual bay scallops are commercially significant bivalve species for fisheries and aquaculture, but their small size and severe inbreeding depression impede the development of their industry. Some interspecific hybrids of bay scallops and peruvian scallops show longer lifespans and significantly greater sizes, which may result from the longevity genes in the latter (7-10 years). Sirtuins (SIRTs) play pivotal roles in the genetic control of aging in various model species and human beings. However, the role of SIRTs in longevity has not been systematically studied in aquatic animals. In this study, different gene numbers, sequences, structures and tandem duplications of SIRTs were first identified between the two scallops through genome-wide analysis. Cloning and characteristics of the SIRT1 and SIRT6 ORFs revealed dramatic variations in amino acids between the two scallops, which may cause intrinsic differences in function for longevity regulation. In particular, the amino acid variations in the N-terminus may auto-regulate conformations, causing intrinsic differences in catalytic activity for longevity regulation. The robust expression of SIRT1 and SIRT6-2 in peruvian scallops suggested they may exert a role in extending the lifespan. Nutrient restriction (NR) could promote lifespan in terrestrial model organisms, and the SIRTs and their related genes responded to NR for longevity in scallops; peruvian scallops showed a higher ability of autophagy. This study provides potential biomarkers for breeding long-lived larger scallop hybrids for the sustainability of aquaculture. Moreover, the genetic variation during evolution in the two scallops provides a foundation for further research on the longevity function of the SIRTs.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-024-00269-3.