评估小鼠离体骨的结构结构、完整性和细胞组成的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-05-24 DOI:10.1016/j.xpro.2025.103843

Jonathan W Lewis, Kathryn Frost, Georgiana Neag, Holly Adams, Emily Powell, Orla Gallagher, Ilaria Bellantuono, Amy J Naylor, Helen M McGettrick

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引用次数: 0

摘要

了解小鼠骨骼的变化对于理解骨稳态和骨疾病治疗的作用至关重要。在这里，我们提出了一个方案，以评估结构结构，完整性和细胞组成的小鼠骨离体。我们描述了通过显微计算机断层扫描（CT）成像和分析骨骼的步骤，并通过三点弯曲功能测试骨骼强度。然后我们详细说明了钙黄蛋白标记的组织学和分析程序。有关该协议使用和执行的完整细节，请参见Lewis等人1。

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Protocol for assessing the structural architecture, integrity, and cellular composition of murine bone ex vivo.

Understanding changes in murine bone is essential to comprehend bone homeostasis and the role of therapeutics in bone diseases. Here, we present a protocol to assess the structural architecture, integrity, and cellular composition of murine bone ex vivo. We describe steps for imaging and analyzing bone by micro-computed tomography (CT) and functionally testing bone strength through three-point bending. We then detail procedures for histology and analysis of calcein labeling. For complete details on the use and execution of this protocol, please refer to Lewis et al.¹.

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