[Nrf2/HO-1通路在氯氰菊酯诱导小鼠海马神经元氧化损伤中的作用]。

Q3 Medicine
Lihua Zhou, Xun Zhang, Yingying Yu, Panpan Zhang
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引用次数: 0

摘要

目的:探讨抗氧化轴Nrf2/HO-1是否参与氯氰菊酯诱导海马损伤的调控及其机制。方法:将10周龄C57BL/6小鼠随机分为对照组和氯氰菊酯低、中、高暴露组。灌胃玉米油(对照)或氯氰菊酯21 d后,检测小鼠海马组织中MDA、T-SOD、GSH-Px和CAT水平,评价氧化应激水平。HE染色观察海马神经元形态学变化。采用Western blotting、免疫荧光染色、RT-qPCR检测Nrf2、HO-1、HO-1蛋白表达及mRNA表达。结果:亚急性口服氯氰菊酯可显著提高C57BL/6小鼠MDA水平,降低抗氧化酶T-SOD、GSH-Px和CAT活性,诱导海马CA1和CA3区神经元损伤。氯氰菊酯暴露还导致Nrf2蛋白从细胞质向细胞核易位,并伴有关键抗氧化因子Nrf2及其下游靶激酶HO-1表达水平上调。结论:氯氰菊酯暴露可引起C57BL/6小鼠海马的氧化损伤,并受Nrf2/HO-1抗氧化通路调控。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Role of the Nrf2/HO-1 pathway in cypermethrin-induced oxidative injury of mice hippocampal neurons].

Objectives: To explore whether the antioxidant axis Nrf2/HO-1 is involved in the regulation of hippocampus injury induced by cypermethrin and its underlying mechanism.

Methods: Ten-week-old C57BL/6 mice were randomly divided into control group and cypermethrin exposure groups with low, medium, and high exposure levels. After 21 days of oral gavage of corn oil (control) or cypermethrin, the levels of MDA, T-SOD, GSH-Px and CAT in the hippocampus of the mice were examined to evaluate the oxidative stress levels. HE staining was used to observe morphological changes of the hippocampal neurons. Western blotting, immunofluorescence staining and RT-qPCR were employed to detect the protein expressions and mRNA expression of Nrf2 and HO-1 and HO-1.

Results: Subacute oral exposure to cypermethrin significantly increased MDA level, decreased the activities of antioxidant enzymes T-SOD, GSH-Px and CAT, and induced neuronal damage in the CA1 and CA3 regions in the hippocampus of C57BL/6 mice. Cypermethrin exposure also caused Nrf2 protein translocation from the cytoplasm to the nucleus, accompanied by upregulated expression levels of the key antioxidant factor Nrf2 and its downstream target kinase HO-1.

Conclusions: Cypermethrin exposure dose-dependently causes oxidative damage in the hippocampus of C57BL/6 mice, which is regulated by the Nrf2/HO-1 antioxidant pathway.

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来源期刊
南方医科大学学报杂志
南方医科大学学报杂志 Medicine-Medicine (all)
CiteScore
1.50
自引率
0.00%
发文量
208
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