在暴露于这种毒物水平的三个队列中,砷暴露相关甲基化变化的协同效应。

IF 2.6 3区 医学 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Katarzyna Ewa Sokolowska, Jacek Antoniewski, Marta Sobalska-Kwapis, Dominik Strapagiel, Wojciech Marciniak, Jan Lubiński, Tomasz Kazimierz Wojdacz
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引用次数: 0

摘要

目的:评估砷暴露对甲基组影响的研究结果在很大程度上不一致。为了帮助理解砷暴露对暴露细胞甲基组的影响,我们评估了低水平砷暴露对三组暴露个体血细胞甲基组的影响。方法:使用Infinium MethylationEPIC阵列(Illumina Inc.)进行全基因组甲基化分析和鲁棒线性回归,以确定12年无癌症随访的健康个体和乳腺癌患者的血细胞中砷相关的甲基化变化,平均在诊断前4.29年采样,以及来自暴露于砷的生物标志物队列脐带血样本的甲基化数据。结果:我们的分析确定了健康个体血液中2,453个砷相关甲基化变化,乳腺癌患者血液中9,662个甲基化变化,脐带血样本中6,745个甲基化变化。与之前的研究类似,我们在每个队列中发现的甲基化变化只是在一定程度上重叠。然而,与确定的甲基化变化相关的分子过程在每个队列中都非常相似。包括与砷暴露的不良影响,特别是癌症患者群体中的癌症明显相关的途径。此外,在每个队列中鉴定的甲基化变化的基因组区域是相似的,主要包括参与基因转录调节的区域。结论:总体而言,我们的研究结果表明,砷相关甲基化变化的特异性很低,但这些变化对细胞生理的影响在我们所研究的三个队列中非常相似。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergic effect of arsenic exposure related methylation changes in three cohorts exposed to levels of this toxicant.

Purpose: The results of studies assessing impact of arsenic exposure on methylome are to large extent inconsistent. To contribute to understanding of effect of arsenic exposure on methylome of the exposed cells, we assess the impact of low-level arsenic exposure on methylome of blood cells in three cohorts of exposed individuals.

Methods: The Infinium MethylationEPIC array (Illumina Inc.) was used for genome-wide methylation profiling and robust linear regression to identify arsenic-related methylation changes in blood cells from healthy individuals with a 12-year cancer-free follow-up and breast cancer patients, sampled on average 4.29 years before diagnosis, as well as methylomics data from cord blood samples of Biomarkers of Exposure to Arsenic cohort.

Results: Our analysis identified a 2,453 arsenic-associated methylation changes in blood from healthy individuals, 9,662 in breast cancer patients and 6,745 in cord blood samples. Similarly to previous studies methylation changes that we identified in each cohort, overlapped only to some extent. However, molecular processes linked to identified methylation changes were very similar in each of the cohorts. And included pathways that could be clearly associated with the adverse effects of arsenic exposure and specifically cancer in the cohort of cancer patients. Moreover, the genomic regions harboring identified in each cohort methylation changes were similar and predominantly included regions participating in regulation of gene transcription.

Conclusion: Overall, our findings show that specificity of arsenic related methylation changes is low but the impact of these changes on cell physiology is very similar across three cohorts we studded.

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来源期刊
International Archives of Occupational and Environmental Health
International Archives of Occupational and Environmental Health 医学-公共卫生、环境卫生与职业卫生
CiteScore
5.60
自引率
6.70%
发文量
127
审稿时长
3 months
期刊介绍: International Archives of Occupational and Environmental Health publishes Editorials, Review Articles, Original Articles, and Letters to the Editor. It welcomes any manuscripts dealing with occupational or ambient environmental problems, with a special interest in research at the interface of occupational health and clinical medicine. The scope ranges from Biological Monitoring to Dermatology, from Fibers and Dust to Human Toxicology, from Nanomaterials and Ultra-fine Dust to Night- and Shift Work, from Psycho-mental Distress and Burnout to Vibrations. A complete list of topics can be found on the right-hand side under For authors and editors. In addition, all papers should be based on present-day standards and relate to: -Clinical and epidemiological studies on morbidity and mortality -Clinical epidemiological studies on the parameters relevant to the estimation of health risks -Human experimental studies on environmental health effects. Animal experiments are only acceptable if relevant to pathogenic aspects. -Methods for studying the topics mentioned above.
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