schlegeles鳃源性细胞系的建立及其在病原菌-宿主相互作用研究中的实用性。

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-05-23 DOI:10.1016/j.fsi.2025.110454

Xin Zhang, Yan Ling Sun, Qiang Fu, Chao Li, Hui Jun Huo

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引用次数: 0

摘要

在本研究中，我们建立并描述了一株来自schlegelii Sebastes鳃的连续细胞系，命名为SSG。该细胞系已连续培养50余代。核型分析证实细胞中存在48条正常的二倍体染色体。COX1和16S rRNA基因测序结果证实了SSG细胞的schlegelii起源。SSG在siRNA探针上表现出中等转染效率，表明其在基因干扰研究中的潜力。感染敏感性评估显示，鱼酸爱德华菌可以在SSG中成功存活和增殖，引发以活性氧（ROS）大量产生和免疫相关基因上调为特征的免疫反应。该体外感染模型为研究schlegelii鳃内病原菌-寄主相互作用机制提供了有效工具，对该物种细菌性疾病的预防和控制具有重要意义。

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Establishment of a gill-derived cell line in Sebastes schlegelii with practicability in studies of bacterial pathogen-host interaction

In this study, we established and described a continuous cell line derived from gill of Sebastes schlegelii, named SSG. This cell line has been successively cultured for over 50 passages. Karyotype analysis confirmed the presence of 48 normal diploid chromosomes in the cells. Sequencing results of COX1 and 16S rRNA genes verified the S. schlegelii origin of SSG cells. SSG demonstrated moderate transfection efficiency with siRNA probes, indicating its potential for gene interference studies. Infection sensitivity assessments revealed that Edwardsiella piscicida can successfully survive and proliferate in SSG, triggering an immune response characterized by the substantial production of reactive oxygen species (ROS) and the upregulation of immune-related genes. This in vitro infection model provides an effective tool for studying bacterial pathogen-host interaction mechanisms in gill of S. schlegelii and holds significant importance for the prevention and control of bacterial diseases in this species.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.