Mustafa Karatepe, Münir Aktaş, Bilge Karatepe, Sezayi Özübek
{"title":"绵羊和山羊无原体种类调查，具有兽医和公共卫生意义","authors":"Mustafa Karatepe, Münir Aktaş, Bilge Karatepe, Sezayi Özübek","doi":"10.1007/s11686-025-01056-5","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>This study was carried out to investigate <i>Anaplasma</i> important for veterinary and public health in sheep and goats in Niğde province in Türkiye by using molecular methods.</p><h3>Methods</h3><p>Blood samples were taken from randomly selected 690 animals (520 sheep and 170 goats), which were between 1 and 10 years old and from different study sites in Niğde by using the vacutainer tubes containing EDTA. After the genomic DNA extractions samples, the <i>Anaplasma</i> spp. 16S rRNA genes were amplified by PCR. Species-specific polymerase chain reaction (PCR) assays were performed on positive samples for the presence of <i>A. bovis</i>, <i>A. capra</i>, <i>A. ovis, A. platys</i>-like, and <i>A. phagocytophilum</i>. At the same time, the animals were tested for ixodid tick infestation and collected ticks were examined for identification under the stereo-microscope.</p><h3>Results</h3><p>The results of PCR analysis show that the overall <i>A. ovis</i> prevalence was 63.3% (437/690) in small ruminants sampled. A total of 361 sheep (69.4%) and 76 goats (44.7%) were found to be infected with <i>A. ovis</i>, whereas no positivity was detected for <i>A. bovis</i>, <i>A. capra</i>, <i>A. platys</i>-like, and <i>A. phagocytophilum. Anaplasma ovis</i> positivity was observed at the highest percent in May (%74.6) while the lowest in June (%52.4). In total, 1361 ticks (579♀, 782♂) were collected from sheep and goats in Niğde. Ticks were identified as <i>Rhipicephalus bursa</i> (383, 28.1%), <i>R. turanicus</i> (607, 44.6%), <i>Hyalomma marginatum</i> (7, 0.5%), <i>Hy. excavatum</i> (247, 18.1%), <i>Hy. anatolicum</i> (23, 1.7%), <i>Haemophsalis parva</i> (21, 1.5%), <i>Hae. punctata</i> (7, 0.5%), <i>Hae. sulcata</i> (40, 2.9%) and <i>Dermacentor marginatus</i> (26, 1.9%).</p><h3>Conclusion</h3><p>The present study reports a high prevalence of <i>A. ovis</i> 63.3% (437/690) in sheep and goats in Niğde province.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-025-01056-5.pdf","citationCount":"0","resultStr":"{\"title\":\"Investigation of Anaplasma Species with Veterinary and Public Health Significance in Sheep and Goats\",\"authors\":\"Mustafa Karatepe, Münir Aktaş, Bilge Karatepe, Sezayi Özübek\",\"doi\":\"10.1007/s11686-025-01056-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>This study was carried out to investigate <i>Anaplasma</i> important for veterinary and public health in sheep and goats in Niğde province in Türkiye by using molecular methods.</p><h3>Methods</h3><p>Blood samples were taken from randomly selected 690 animals (520 sheep and 170 goats), which were between 1 and 10 years old and from different study sites in Niğde by using the vacutainer tubes containing EDTA. After the genomic DNA extractions samples, the <i>Anaplasma</i> spp. 16S rRNA genes were amplified by PCR. Species-specific polymerase chain reaction (PCR) assays were performed on positive samples for the presence of <i>A. bovis</i>, <i>A. capra</i>, <i>A. ovis, A. platys</i>-like, and <i>A. phagocytophilum</i>. At the same time, the animals were tested for ixodid tick infestation and collected ticks were examined for identification under the stereo-microscope.</p><h3>Results</h3><p>The results of PCR analysis show that the overall <i>A. ovis</i> prevalence was 63.3% (437/690) in small ruminants sampled. A total of 361 sheep (69.4%) and 76 goats (44.7%) were found to be infected with <i>A. ovis</i>, whereas no positivity was detected for <i>A. bovis</i>, <i>A. capra</i>, <i>A. platys</i>-like, and <i>A. phagocytophilum. Anaplasma ovis</i> positivity was observed at the highest percent in May (%74.6) while the lowest in June (%52.4). In total, 1361 ticks (579♀, 782♂) were collected from sheep and goats in Niğde. Ticks were identified as <i>Rhipicephalus bursa</i> (383, 28.1%), <i>R. turanicus</i> (607, 44.6%), <i>Hyalomma marginatum</i> (7, 0.5%), <i>Hy. excavatum</i> (247, 18.1%), <i>Hy. anatolicum</i> (23, 1.7%), <i>Haemophsalis parva</i> (21, 1.5%), <i>Hae. punctata</i> (7, 0.5%), <i>Hae. sulcata</i> (40, 2.9%) and <i>Dermacentor marginatus</i> (26, 1.9%).</p><h3>Conclusion</h3><p>The present study reports a high prevalence of <i>A. ovis</i> 63.3% (437/690) in sheep and goats in Niğde province.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"70 3\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s11686-025-01056-5.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-025-01056-5\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-025-01056-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Investigation of Anaplasma Species with Veterinary and Public Health Significance in Sheep and Goats
Purpose
This study was carried out to investigate Anaplasma important for veterinary and public health in sheep and goats in Niğde province in Türkiye by using molecular methods.
Methods
Blood samples were taken from randomly selected 690 animals (520 sheep and 170 goats), which were between 1 and 10 years old and from different study sites in Niğde by using the vacutainer tubes containing EDTA. After the genomic DNA extractions samples, the Anaplasma spp. 16S rRNA genes were amplified by PCR. Species-specific polymerase chain reaction (PCR) assays were performed on positive samples for the presence of A. bovis, A. capra, A. ovis, A. platys-like, and A. phagocytophilum. At the same time, the animals were tested for ixodid tick infestation and collected ticks were examined for identification under the stereo-microscope.
Results
The results of PCR analysis show that the overall A. ovis prevalence was 63.3% (437/690) in small ruminants sampled. A total of 361 sheep (69.4%) and 76 goats (44.7%) were found to be infected with A. ovis, whereas no positivity was detected for A. bovis, A. capra, A. platys-like, and A. phagocytophilum. Anaplasma ovis positivity was observed at the highest percent in May (%74.6) while the lowest in June (%52.4). In total, 1361 ticks (579♀, 782♂) were collected from sheep and goats in Niğde. Ticks were identified as Rhipicephalus bursa (383, 28.1%), R. turanicus (607, 44.6%), Hyalomma marginatum (7, 0.5%), Hy. excavatum (247, 18.1%), Hy. anatolicum (23, 1.7%), Haemophsalis parva (21, 1.5%), Hae. punctata (7, 0.5%), Hae. sulcata (40, 2.9%) and Dermacentor marginatus (26, 1.9%).
Conclusion
The present study reports a high prevalence of A. ovis 63.3% (437/690) in sheep and goats in Niğde province.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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