ERF转录因子OpCBR1平衡蛇根生长和抗癌药物喜树碱的生物合成

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-05-27 DOI:10.1016/j.indcrop.2025.121259

Qin Zhou , Yongpeng Li , Yiqing Peng , Jingyi Wang , Xiaoxuan Fan , Liqin Li , Miaomiao Sheng , Yao Wang , Xiaolong Hao , Guoyin Kai

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引用次数: 0

摘要

喜树碱是一种广泛用于治疗各种癌症的化疗药物。蛇根是中国的一种民间药物，已经发展成为研究喜树碱生物合成途径及其调控的理想模式植物。乙烯反应因子（ERF）转录因子在植物界广泛存在，并且被认为是调节次生代谢的。然而，它们在喜树碱生产中的作用尚不清楚。本研究共鉴定出78个OpERF基因，其中发现OpCBR1在调节喜树碱生物合成和毛状根生长中具有双重作用。过表达OpCBR1抑制喜树碱的生物合成，但促进树根生长，反之，敲除OpCBR1则产生相反的效果。进一步分析发现，OpCBR1直接抑制OpIO启动子，从而减少喜树碱的积累。同时，通过上调生长素和细胞分裂素相关基因来提高生物量，为通过生物质工程提高喜树碱产量提供了一种遗传途径。本研究强调ERF转录因子是植物代谢和发育的重要调控因子。

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The ERF transcription factor OpCBR1 balances root growth and biosynthesis of the anticancer drug camptothecin in Ophiorrhiza pumila

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The ERF transcription factor OpCBR1 balances root growth and biosynthesis of the anticancer drug camptothecin in Ophiorrhiza pumila

Camptothecin is a chemotherapeutic drug widely used to treat various cancers. Ophiorrhiza pumila, a folk medicine in China, has evolved as an ideal model plant for studying the biosynthetic pathway of camptothecin as well as its regulation. Ethylene-responsive factor (ERF) transcription factors are conservatively widespread in the plant kingdom, and are well known to regulate secondary metabolism. However, their involvement in camptothecin production remains unclear. In this study, a total of 78 OpERF genes were identified in O. pumila, among which, OpCBR1 was found to have dual roles in regulating camptothecin biosynthesis and hairy root growth. Overexpression of OpCBR1 suppressed camptothecin biosynthesis but promoted root growth, conversely, its knockout produced opposite effects. Further analysis revealed OpCBR1 directly represses the OpIO promoter to reduce camptothecin accumulation. Meanwhile, it upregulates auxin- and cytokinin-related genes to enhance biomass, providing a genetic approach to improve camptothecin yield through biomass engineering. This study highlights ERF transcription factors as important regulators of plant metabolism and development.

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.