Investigating the effect of Lactobacillus crispatus extract on the expression of Axin2 and GSK3β genes from Wnt pathway in uterine cell line

Introduction and aim

The Wnt1 signaling pathway is recognized for its involvement in cell growth as well as its capacity to trigger apoptosis. A variety of research has underscored the effect of Lactobacillus populations on uterine health, especially in the context of embryo implantation. This study seeks to explore how bacillus extracts affect the expression of Wnt1 genes in the context of recurrent miscarriage, with a particular emphasis on endometrial stem cells (ENSC).

Material and method

A preparation of Lactobacillus crispatus extract was created, and the ENSC cell line was grown in specialized media containing different concentrations of the bacterial extract and subjected to various incubation times. Total RNA was isolated from the cultured cells, which was then used to synthesize cDNA. Real-time PCR was performed with specific primer pairs targeting Axin2 and GSK3β, in addition to amplifying a suitable housekeeping gene for uterine tissue. The results were evaluated using SPSS and LinReg software.

Results

The application of the crispatus extract to the ENSC cell line led to a notable increase in the expression of Axin2, whereas GSK3β demonstrated a non-significant rise within the Wnt1 pathway (p value = 0.001). It was observed that the bacterial extract displayed a quantifiable level of cytotoxicity at elevated concentrations. The findings revealed that the expression of the Axin2 gene was significantly greater than that of GSK3β (p value = 0.001). No significant changes in gene expression were observed in the untreated ENSC cell lines.

Conclusion

A more comprehensive insight into the effects of bacterial extracts on the active genes involved in biochemical pathways within endometrial tissue could aid in the formulation of probiotic approaches and therapies for recurrent miscarriage, as these genes play a significant role in this condition.