富氢水通过抑制乙烯和ABA水平，从而减少叶绿素降解和类胡萝卜素积累，延缓西兰花收获后的黄变

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-05-27 DOI:10.1016/j.postharvbio.2025.113661

Yi Wang , Xintong Chen , Wei Chen , Zhenfeng Yang , Liyu Shi , Xuewen Li , Shifeng Cao , Wei Song

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引用次数: 0

摘要

为探讨花椰菜黄变的机理和防治方法，本研究以富氢水（HRW）处理对花椰菜黄变的影响及机理为研究对象。研究结果表明，HRW处理维持了西兰花采后贮藏过程中叶绿体结构的完整性，减缓了叶绿素的降解和类胡萝卜素的积累。RT-qPCR结果显示，HRW处理抑制了叶绿素降解基因BoNOL1/6/10、BoPAO、BoPOX、BoSGR1、BoCLH1、BoNCY、BoNYC以及类胡萝卜素相关生物合成基因BoPSY、BoPDS、BoZDS、BoZISO、BoCRTISO、BoCYP97C1、BoCCS-1的表达，提高了BoCCD1/4的表达水平。此外，HRW处理通过下调其生物合成基因BoACO1/2和BoACS1/2/7来抑制内源乙烯的产生。同时，HRW处理下调了ABA生物合成基因BoNCED2/3和BoABA2，导致ABA水平显著降低。综上所述，HRW处理通过抑制乙烯和ABA水平延缓了西兰花收获后的黄变，从而减少了叶绿素的降解和类胡萝卜素的积累。

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Hydrogen-rich water delays post-harvest yellowing in broccoli by inhibiting ethylene and ABA levels, thereby reducing chlorophyll degradation and carotenoid accumulation

To investigate the mechanism of yellowing and explore control methods, this study focused on the impact and mechanism of hydrogen-rich water (HRW) treatment on broccoli floret yellowing. The research results indicate that HRW treatment maintains the structural integrity of chloroplasts in broccoli florets during post-harvest storage, slowing down the degradation of chlorophyll and the accumulation of carotenoids. RT-qPCR results show that HRW treatment inhibits the expression of chlorophyll degradation genes BoNOL1/6/10, BoPAO, BoPOX, BoSGR1, BoCLH1, BoNCY, and BoNYC, as well as carotenoid-related biosynthesis genes BoPSY, BoPDS, BoZDS, BoZISO, BoCRTISO, BoCYP97C1, and BoCCS-1, while increasing the expression level of BoCCD1/4. Furthermore, HRW treatment inhibits the production of endogenous ethylene through downregulation of its biosynthetic genes BoACO1/2 and BoACS1/2/7. Simultaneously, HRW treatment downregulates ABA biosynthesis genes BoNCED2/3 and BoABA2, resulting in a significant reduction in ABA levels. In summary, the HRW treatment delays the yellowing of broccoli after harvest by inhibiting ethylene and ABA levels, thereby reducing the degradation of chlorophyll and the accumulation of carotenoids.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.