{"title":"急性髓系白血病易感性:白细胞介素37和38遗传变异的影响","authors":"Mustafa A. Bashi , Ali H. Ad’hiah","doi":"10.1016/j.cca.2025.120386","DOIUrl":null,"url":null,"abstract":"<div><div>Interleukin (IL)-37 and IL-38 are anti-inflammatory cytokines encoded by <em>IL37</em> and <em>IL1F10</em> genes, respectively. Recently, it has been proposed that missense single nucleotide polymorphisms (SNPs) of <em>IL37</em> (rs3811046 G/T and rs3811047 A/G) and 5′-untranslated region SNPs of <em>IL1F10</em> (rs3811050 C/T and rs3811051 T/G) may influence susceptibility to some inflammatory disorders. Inflammation has also been shown to play a key role in pathogenesis of acute myeloid leukemia (AML). Therefore, it is reasonable to hypothesize that rs3811046, rs3811047, rs3811050, and rs3811051 may have a role in susceptibility to AML. A case-control study was conducted on 131 AML patients and 169 controls to investigate the association of these SNPs with AML risk. SNP genotypes were determined using TaqMan allelic discrimination, a real-time PCR-based method. Results revealed that mutant alleles (<em>T</em>, <em>G</em>, and <em>T</em>) and corresponding homozygous genotypes (TT, GG, and TT) of rs3811046, rs3811047, and rs3811050, respectively, were significantly associated with an increased risk of AML, while rs3811051 showed no association. Four-locus haplotype analysis (rs3811046-rs3811047-rs3811050-rs3811051) demonstrated that T-A-C-G haplotype was associated a 2.47-fold increased risk of AML. SNP-SNP interaction analysis showed significant genetic interactions between rs3811046 and rs3811047, rs3811046 and rs3811050, and rs3811047 and rs3811050. Rs3811046 and rs3811047 did not affect <em>IL37</em> gene expression. Some laboratory and clinical variables of AML may be influenced by <em>IL37</em> and <em>IL1F10</em> SNPs. In conclusion, rs3811046, rs3811047, and rs3811050 were associated with AML susceptibility in terms of allele, genotype, and haplotype, while rs3811051 showed no association. Three SNPs (rs3811046, rs3811047, and rs3811050) showed a significant gene-gene interaction.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120386"},"PeriodicalIF":2.9000,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Susceptibility to acute myeloid leukemia: Influence of genetic variants of interleukins 37 and 38\",\"authors\":\"Mustafa A. Bashi , Ali H. Ad’hiah\",\"doi\":\"10.1016/j.cca.2025.120386\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Interleukin (IL)-37 and IL-38 are anti-inflammatory cytokines encoded by <em>IL37</em> and <em>IL1F10</em> genes, respectively. Recently, it has been proposed that missense single nucleotide polymorphisms (SNPs) of <em>IL37</em> (rs3811046 G/T and rs3811047 A/G) and 5′-untranslated region SNPs of <em>IL1F10</em> (rs3811050 C/T and rs3811051 T/G) may influence susceptibility to some inflammatory disorders. Inflammation has also been shown to play a key role in pathogenesis of acute myeloid leukemia (AML). Therefore, it is reasonable to hypothesize that rs3811046, rs3811047, rs3811050, and rs3811051 may have a role in susceptibility to AML. A case-control study was conducted on 131 AML patients and 169 controls to investigate the association of these SNPs with AML risk. SNP genotypes were determined using TaqMan allelic discrimination, a real-time PCR-based method. Results revealed that mutant alleles (<em>T</em>, <em>G</em>, and <em>T</em>) and corresponding homozygous genotypes (TT, GG, and TT) of rs3811046, rs3811047, and rs3811050, respectively, were significantly associated with an increased risk of AML, while rs3811051 showed no association. Four-locus haplotype analysis (rs3811046-rs3811047-rs3811050-rs3811051) demonstrated that T-A-C-G haplotype was associated a 2.47-fold increased risk of AML. SNP-SNP interaction analysis showed significant genetic interactions between rs3811046 and rs3811047, rs3811046 and rs3811050, and rs3811047 and rs3811050. Rs3811046 and rs3811047 did not affect <em>IL37</em> gene expression. Some laboratory and clinical variables of AML may be influenced by <em>IL37</em> and <em>IL1F10</em> SNPs. In conclusion, rs3811046, rs3811047, and rs3811050 were associated with AML susceptibility in terms of allele, genotype, and haplotype, while rs3811051 showed no association. Three SNPs (rs3811046, rs3811047, and rs3811050) showed a significant gene-gene interaction.</div></div>\",\"PeriodicalId\":10205,\"journal\":{\"name\":\"Clinica Chimica Acta\",\"volume\":\"576 \",\"pages\":\"Article 120386\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinica Chimica Acta\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0009898125002657\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinica Chimica Acta","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009898125002657","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Susceptibility to acute myeloid leukemia: Influence of genetic variants of interleukins 37 and 38
Interleukin (IL)-37 and IL-38 are anti-inflammatory cytokines encoded by IL37 and IL1F10 genes, respectively. Recently, it has been proposed that missense single nucleotide polymorphisms (SNPs) of IL37 (rs3811046 G/T and rs3811047 A/G) and 5′-untranslated region SNPs of IL1F10 (rs3811050 C/T and rs3811051 T/G) may influence susceptibility to some inflammatory disorders. Inflammation has also been shown to play a key role in pathogenesis of acute myeloid leukemia (AML). Therefore, it is reasonable to hypothesize that rs3811046, rs3811047, rs3811050, and rs3811051 may have a role in susceptibility to AML. A case-control study was conducted on 131 AML patients and 169 controls to investigate the association of these SNPs with AML risk. SNP genotypes were determined using TaqMan allelic discrimination, a real-time PCR-based method. Results revealed that mutant alleles (T, G, and T) and corresponding homozygous genotypes (TT, GG, and TT) of rs3811046, rs3811047, and rs3811050, respectively, were significantly associated with an increased risk of AML, while rs3811051 showed no association. Four-locus haplotype analysis (rs3811046-rs3811047-rs3811050-rs3811051) demonstrated that T-A-C-G haplotype was associated a 2.47-fold increased risk of AML. SNP-SNP interaction analysis showed significant genetic interactions between rs3811046 and rs3811047, rs3811046 and rs3811050, and rs3811047 and rs3811050. Rs3811046 and rs3811047 did not affect IL37 gene expression. Some laboratory and clinical variables of AML may be influenced by IL37 and IL1F10 SNPs. In conclusion, rs3811046, rs3811047, and rs3811050 were associated with AML susceptibility in terms of allele, genotype, and haplotype, while rs3811051 showed no association. Three SNPs (rs3811046, rs3811047, and rs3811050) showed a significant gene-gene interaction.
期刊介绍:
The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)
Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells.
The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.